Expression CD40L on mouse platelets detected by flow cytometry
. | . | CD40L expression . | . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse strain . | No. mice . | Resting platelets . | Platelets incubated with O157LPS . | P* . | Thrombin-stimulated platelets . | P* . | Resting platelets preincubated with 10 μg/mL anti-CD62 and O157 LPS . | P† . | Resting platelets preincubated with 50 μg/mL anti-CD 62 and O157 LPS . | P† . | ||||||||
| C57BL/10ScSn | 10 | 1.7 (0.1-3.7) | 45.4 (25.6-75.9) | .001 | 59.3 (42.6-72.6) | .053‡ | 39.8 (35.2-65.5) | .631‡ | 17.2 (9.1-39.4) | .001 | ||||||||
| C57BL/10ScN | 5 | 4.0 (3.0-4.1) | 0.14 (0.10-0.35) | NA | 49.5 (45.9-65.8) | NA | 1.04 (0.68-1.54) | .197‡ | NA | NA | ||||||||
| C3H/HeN | 10 | 0.3 (0.2-1.1) | 45.3 (25.6-81.6) | .001 | 54.5 (18.2-72.8) | .490‡ | 32.5 (17.6-49.2) | .393‡ | 21.2 (10.2-34.1) | .001 | ||||||||
| C3H/HeJ | 5 | 0.3 (0.1-0.9) | 0.99 (0.01-1.49) | NA | 49.3 (30.7-63.0) | NA | 0.29 (0.08-6.53) | .548‡ | NA | NA | ||||||||
. | . | CD40L expression . | . | . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse strain . | No. mice . | Resting platelets . | Platelets incubated with O157LPS . | P* . | Thrombin-stimulated platelets . | P* . | Resting platelets preincubated with 10 μg/mL anti-CD62 and O157 LPS . | P† . | Resting platelets preincubated with 50 μg/mL anti-CD 62 and O157 LPS . | P† . | ||||||||
| C57BL/10ScSn | 10 | 1.7 (0.1-3.7) | 45.4 (25.6-75.9) | .001 | 59.3 (42.6-72.6) | .053‡ | 39.8 (35.2-65.5) | .631‡ | 17.2 (9.1-39.4) | .001 | ||||||||
| C57BL/10ScN | 5 | 4.0 (3.0-4.1) | 0.14 (0.10-0.35) | NA | 49.5 (45.9-65.8) | NA | 1.04 (0.68-1.54) | .197‡ | NA | NA | ||||||||
| C3H/HeN | 10 | 0.3 (0.2-1.1) | 45.3 (25.6-81.6) | .001 | 54.5 (18.2-72.8) | .490‡ | 32.5 (17.6-49.2) | .393‡ | 21.2 (10.2-34.1) | .001 | ||||||||
| C3H/HeJ | 5 | 0.3 (0.1-0.9) | 0.99 (0.01-1.49) | NA | 49.3 (30.7-63.0) | NA | 0.29 (0.08-6.53) | .548‡ | NA | NA | ||||||||
Expression of CD40L is shown as median and range values of experiments. To inhibit the CD62 phenotype, platelets were preincubated with rat anti-mouse CD62 antibody (10 μg/mL or 50 μg/mL) or rat IgG (at the same concentration), followed by incubation with O157LPS (diluted in autologous plasma).
NA indicates not analyzed.
P values evaluated by comparison of platelets from wild-type mice with those of mutated mice.
P values evaluated by comparison of resting platelets incubated with LPS or LPS and anti-CD62. Rat IgG did not block the CD62 phenotype (results not shown).
Not significant.