LPS-induced platelet activation markers and binding of fibrinogen to human platelets detected by flow cytometry
. | Median GPIIb/IIIa activation PAC-1 antibody binding* . | . | . | . | Median CD40L expression median* . | . | . | . | Median fibrinogen binding media* . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Serotype . | Without LPS . | With LPS . | Thrombin . | P† . | Without LPS . | With LPS . | Thrombin . | P† . | Without LPS . | With LPS . | P† . | ||||||||
| O157:H7 | 0.03 (0.01-0.12) | 55.8 (49.2-61.2) | 60.3 (55.2-63.4) | .005 | 0.05 (0.01-0.23) | 58.1 (38.8-64.5) | 62.6 (57.7-66.1) | <.001 | 0.12 (0.07-0.18) | 97.1 (43.0-99.1) | <.0001 | ||||||||
| O103:H2 | 0.07 (0.03-0.11) | 15.4 (3.3-39.3) | 70.5 (68.5-73.2) | .008 | 0.10 (0.04-0.15) | 13.1 (2.7-17.8) | 65.4 (60.9-68.2) | .009 | NT | NT | — | ||||||||
| O111:HN | 0.05 (0.04-0.07) | 17.3 (4.2-27.1) | 63.9 (60.0-65.7) | .008 | 0.12 (0.02-0.20) | 15.2 (6.1-23.3) | 66.2 (60.6-73.4) | .009 | NT | NT | — | ||||||||
| O121:H19 | 0.12 (0.09-0.15) | 21.9 (8.9-61.3) | 67.7 (62.1-70.3) | .006 | 0.04 (0.01-0.07) | 18.7 (13.4-20.6) | 61.3 (58.8-63.9) | .008 | 0.24 (0.10-0.40) | 27.6 (19.4-38.7) | .005 | ||||||||
. | Median GPIIb/IIIa activation PAC-1 antibody binding* . | . | . | . | Median CD40L expression median* . | . | . | . | Median fibrinogen binding media* . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Serotype . | Without LPS . | With LPS . | Thrombin . | P† . | Without LPS . | With LPS . | Thrombin . | P† . | Without LPS . | With LPS . | P† . | ||||||||
| O157:H7 | 0.03 (0.01-0.12) | 55.8 (49.2-61.2) | 60.3 (55.2-63.4) | .005 | 0.05 (0.01-0.23) | 58.1 (38.8-64.5) | 62.6 (57.7-66.1) | <.001 | 0.12 (0.07-0.18) | 97.1 (43.0-99.1) | <.0001 | ||||||||
| O103:H2 | 0.07 (0.03-0.11) | 15.4 (3.3-39.3) | 70.5 (68.5-73.2) | .008 | 0.10 (0.04-0.15) | 13.1 (2.7-17.8) | 65.4 (60.9-68.2) | .009 | NT | NT | — | ||||||||
| O111:HN | 0.05 (0.04-0.07) | 17.3 (4.2-27.1) | 63.9 (60.0-65.7) | .008 | 0.12 (0.02-0.20) | 15.2 (6.1-23.3) | 66.2 (60.6-73.4) | .009 | NT | NT | — | ||||||||
| O121:H19 | 0.12 (0.09-0.15) | 21.9 (8.9-61.3) | 67.7 (62.1-70.3) | .006 | 0.04 (0.01-0.07) | 18.7 (13.4-20.6) | 61.3 (58.8-63.9) | .008 | 0.24 (0.10-0.40) | 27.6 (19.4-38.7) | .005 | ||||||||
Platelets were incubated with LPS 1 μg/mL (diluted in donor plasma), washed, and incubated with the respective antibody. Percentage binding to the population is shown as median and range values from 5 different experiments. Washed thrombin-stimulated platelets were used as positive control for PAC-1 and α-CD40L binding.
NT indicates not tested;—, not applicable.
Values other than in P columns are expressed as % (range).
P values were evaluated by comparison of resting platelets incubated with or without LPS.