LPS binding to mouse platelets: the effect of anti-CD62 on binding
Strain . | Median LPS binding without thrombin, % (range) . | P . | Median LPS binding with thrombin, % (range) . | P . | LPS binding to thrombin-activated WPs preincubated with 10 μg/mL anti-CD62, % (range) . | LPS binding to thrombin-activated WPs preincubated with 50 μg/mL anti-CD62, % (range) . |
|---|---|---|---|---|---|---|
| C57BL/10ScSn* | 58.2 (30.5-70.3) | <.001 | 63.7 (30.4-77.5) | <.001 | 54.6 (40.0-67.2) | 16.2 (6.9-26.6) |
| C57BL/10ScN* | 0.7 (0.1-7.5) | — | 40.5 (30.4-48.7) | — | 36.3 (31.2-45.7) | NT |
| C3H/HeN† | 71.3 (52.3-88.6) | .841‡ | 70.2 (58.6-89.9) | .055‡ | 51.6 (17.0-57.6) | 7.1 (5.3-9.5) |
| C3H/HeJ† | 61.9 (52.3-79.1) | — | 58.8 (45.1-68.8) | — | 33.7 (10.6-34.8) | NT |
Strain . | Median LPS binding without thrombin, % (range) . | P . | Median LPS binding with thrombin, % (range) . | P . | LPS binding to thrombin-activated WPs preincubated with 10 μg/mL anti-CD62, % (range) . | LPS binding to thrombin-activated WPs preincubated with 50 μg/mL anti-CD62, % (range) . |
|---|---|---|---|---|---|---|
| C57BL/10ScSn* | 58.2 (30.5-70.3) | <.001 | 63.7 (30.4-77.5) | <.001 | 54.6 (40.0-67.2) | 16.2 (6.9-26.6) |
| C57BL/10ScN* | 0.7 (0.1-7.5) | — | 40.5 (30.4-48.7) | — | 36.3 (31.2-45.7) | NT |
| C3H/HeN† | 71.3 (52.3-88.6) | .841‡ | 70.2 (58.6-89.9) | .055‡ | 51.6 (17.0-57.6) | 7.1 (5.3-9.5) |
| C3H/HeJ† | 61.9 (52.3-79.1) | — | 58.8 (45.1-68.8) | — | 33.7 (10.6-34.8) | NT |
Blood was drawn through retro-orbital venous sampling into tubes containing 0.129 M sodium citrate. PRP was obtained by centrifugation at 270g for 3 minutes. Washed thrombin-stimulated or unstimulated platelets were diluted to 108/mL in autologous plasma and incubated with O157LPS 1 μg/mL, fixed, washed, and incubated with monovalent O rabbit antiserum O157-O56. LPS binding to thrombin-activated washed platelets was decreased by preincubation of the platelets with anti-mouse CD62 antibody.
WPs indicates washed platelets; wt, wild-type;—, not applicable; and NT, not tested.
P values were evaluated by comparisons of platelets from wild-type mice and mutant mice.
n - 22 mice.
n = 31 mice.
Not significant.