Cross-competition experiments between polyclonal or monoclonal anti-CD62 antibody and O157LPS
. | Detection of O157LPS binding . | . | . | Detection of anti-CD62 binding . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | OL157 LPS without anti-CD62 . | Simultaneous incubation of anti-CD62 and LPS . | P . | CD62 expression without OL157 LPS . | Simultaneous incubation of anti-CD62 and LPS . | P . | ||||
| Polyclonal α-CD62 | 91.0 (84.3-95.1)* | 3.0 (1.8-3.7) | .008 | 87.0 (82.7-97.3) | 6.5 (4.9-12.2) | .008 | ||||
| Monoclonal α-CD62 | 77.1 (51.3-99.9)* | 69.4 (47.4-91.2) | .548† | 53.2 (47.9-61.5) | 4.0 (1.0-5.2) | .008 | ||||
. | Detection of O157LPS binding . | . | . | Detection of anti-CD62 binding . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | OL157 LPS without anti-CD62 . | Simultaneous incubation of anti-CD62 and LPS . | P . | CD62 expression without OL157 LPS . | Simultaneous incubation of anti-CD62 and LPS . | P . | ||||
| Polyclonal α-CD62 | 91.0 (84.3-95.1)* | 3.0 (1.8-3.7) | .008 | 87.0 (82.7-97.3) | 6.5 (4.9-12.2) | .008 | ||||
| Monoclonal α-CD62 | 77.1 (51.3-99.9)* | 69.4 (47.4-91.2) | .548† | 53.2 (47.9-61.5) | 4.0 (1.0-5.2) | .008 | ||||
Washed thrombin-stimulated platelets were incubated with O157LPS (1 μg/mL, diluted in donor plasma) or anti-human CD62 (rabbit or mouse) or were simultaneously incubated with O157LPS and anti-CD62 (diluted in donor plasma). Incubation of platelets with polyclonal anti-CD62 and LPS reduced LPS binding by 97% and CD62 expression by 93%, respectively. Simultaneous incubation of platelets with the monoclonal antibody and LPS reduced LPS binding minimally and CD62 expression by 92%.
Differences in baseline levels of anti-LPS binding because of donor platelet variations.
Not significant.