Technologies available for identifying and quantifying BCR-ABL KD mutations
Technology . | Sensitivity, % . | Specificity . | Bias* . | Availability . | Reference . |
|---|---|---|---|---|---|
| Direct sequencing | 15-25 | ++ | No | +++ | 19,21,22,24,59 |
| Subcloning and sequencing | 9 | +++ | No | ++ | 20 |
| Denaturing high-performance liquid chromatography (D-HPLC) | 0.1-10 | ++ | No | ++ | 66-68 |
| Pyrosequencing | 5 | ++ | No | + | 55,69 |
| Double-gradient denaturing electrophoresis | 5 | ++ | No | + | 57 |
| Fluorescence PCR and PNA clamping | 0.2 | ++ | Yes | + | 58 |
| Allele-specific oligonucleotide PCR (ASO-PCR) | 0.01 | ++ | Yes | + | 53,59,60 |
Technology . | Sensitivity, % . | Specificity . | Bias* . | Availability . | Reference . |
|---|---|---|---|---|---|
| Direct sequencing | 15-25 | ++ | No | +++ | 19,21,22,24,59 |
| Subcloning and sequencing | 9 | +++ | No | ++ | 20 |
| Denaturing high-performance liquid chromatography (D-HPLC) | 0.1-10 | ++ | No | ++ | 66-68 |
| Pyrosequencing | 5 | ++ | No | + | 55,69 |
| Double-gradient denaturing electrophoresis | 5 | ++ | No | + | 57 |
| Fluorescence PCR and PNA clamping | 0.2 | ++ | Yes | + | 58 |
| Allele-specific oligonucleotide PCR (ASO-PCR) | 0.01 | ++ | Yes | + | 53,59,60 |
PNA indicates peptide nucleic acid.
Bias indicates that the test is designed to detect specific mutations.