Comparison of the phenotype of FLT3-JM-PM– to FLT3-ITD/TKD–expressing Ba/F3 cells
. | FLT3-WT . | FLT3-ITD . | FLT3-TKD . | FLT3-JM-PM . |
|---|---|---|---|---|
| IL-3-independent proliferation* | – | +++ | ++ | + |
| Antiapoptotic activity† | – | +++ | +++ | + |
| Receptor autophosphorylation‡ | – | ++ | ++ | + |
| STAT5 autoactivation§ | – | +++ | +++ | + |
. | FLT3-WT . | FLT3-ITD . | FLT3-TKD . | FLT3-JM-PM . |
|---|---|---|---|---|
| IL-3-independent proliferation* | – | +++ | ++ | + |
| Antiapoptotic activity† | – | +++ | +++ | + |
| Receptor autophosphorylation‡ | – | ++ | ++ | + |
| STAT5 autoactivation§ | – | +++ | +++ | + |
+++ indicates 80%-100%; ++, 50%-79%; +, 5%-49%; and –, less than 5% viable cells in percentage of IL-3+ growth after 72 hours (Figure 2A)
+++ indicates less than 10%; ++, 10%-24%; +, 25%-50%; and –, more than 50% apoptotic cells after 48 hours (Figure 3A)
+++ indicates more than 70%; ++, 40%-70%; +, 10%-39%; and –, less than 10% phospho-FLT3 of total FLT3 (Figure 4B)
+++ indicates 50% or more; ++, 36%-49%; +, 16%-35%; and –, 15% or less phospho-STAT5 of total STAT5 (Figure 5B)