Induction of apoptosis by PGZ and TZD18 in Ph+ ALL cell lines
Cell line and treatment (dose, μM) . | TUNEL, % positive, mean ± SD . | Enrichment factor, mean ± SD* . |
|---|---|---|
| BV173 | ||
| Control | 4.4 ± 0.17 | 1.00 ± 0.05 |
| PGZ (20) | 10.2 ± 0.47 | 1.96 ± 0.11 |
| TZD18 (10) | 7.6 ± 0.76 | 2.15 ± 0.06 |
| TZD18 (20) | 29.2 ± 6.5 | 9.46 ± 0.27 |
| SD1 | ||
| Control | 3.4 ± 0.85 | 1.00 ± 0.06 |
| PGZ (20) | 4.8 ± 1.56 | 1.01 ± 0.06 |
| TZD18 (10) | 9.2 ± 1.84 | 1.53 ± 0.09 |
| TZD18 (20) | 21.7 ± 1.89 | 8.24 ± 0.25 |
| Sup B-15 | ||
| Control | ND | 1.00 ± 0.01 |
| PGZ (20) | ND | ND |
| TZD18 (10) | ND | 1.91 ± 0.14 |
| TZD18 (30) | ND | 2.36 ± 0.07 |
Cell line and treatment (dose, μM) . | TUNEL, % positive, mean ± SD . | Enrichment factor, mean ± SD* . |
|---|---|---|
| BV173 | ||
| Control | 4.4 ± 0.17 | 1.00 ± 0.05 |
| PGZ (20) | 10.2 ± 0.47 | 1.96 ± 0.11 |
| TZD18 (10) | 7.6 ± 0.76 | 2.15 ± 0.06 |
| TZD18 (20) | 29.2 ± 6.5 | 9.46 ± 0.27 |
| SD1 | ||
| Control | 3.4 ± 0.85 | 1.00 ± 0.06 |
| PGZ (20) | 4.8 ± 1.56 | 1.01 ± 0.06 |
| TZD18 (10) | 9.2 ± 1.84 | 1.53 ± 0.09 |
| TZD18 (20) | 21.7 ± 1.89 | 8.24 ± 0.25 |
| Sup B-15 | ||
| Control | ND | 1.00 ± 0.01 |
| PGZ (20) | ND | ND |
| TZD18 (10) | ND | 1.91 ± 0.14 |
| TZD18 (30) | ND | 2.36 ± 0.07 |
BV173, SD1, and Sup B-15 cells were treated with PGZ (20 μM) or TZD18 (10 μM and 20 μM) for 4 days. Apoptosis was determined by TUNEL assay and cell death ELISAplus kit, as described in “Materials and methods.” TUNEL assay results are expressed as percentage of positive cells. Cell death ELISA results are expressed as fold change of enrichment of nucleosomes in the cytoplasm of cells treated with drugs compared with controls (without treatment). Data represent the mean ± SD of triplicate experiments.
ND indicates not done.
Enrichment factor is the enrichment of nucleosomes in the cytoplasm measured by cell death ELISA and is an indicator of apoptosis. Values represent fold change over control