Table 1.

PCR detection of HTLV-1 proviral sequences in inoculated rabbit PBMCs

Inoculum and rabbits
Weeks after inoculation*
2
4
6
8
HTLV-1     
   R1   +   +   +   +  
   R2   +   +   +   +  
   R3   +   +   +   +  
   R4   +   +   +   +  
   R5   +   +   +   +  
HTLV-IΔPBM     
   R39   –   –   –   –  
   R40   –   +   –   –  
   R41   –   +   +   –  
   R42   –   –   –   –  
   R43   –   –   –   –  
729 control     
   R18
 		 –
 		 –
 		 –
 		 –
 
Inoculum and rabbits
Weeks after inoculation*
2
4
6
8
HTLV-1     
   R1   +   +   +   +  
   R2   +   +   +   +  
   R3   +   +   +   +  
   R4   +   +   +   +  
   R5   +   +   +   +  
HTLV-IΔPBM     
   R39   –   –   –   –  
   R40   –   +   –   –  
   R41   –   +   +   –  
   R42   –   –   –   –  
   R43   –   –   –   –  
729 control     
   R18
 		 –
 		 –
 		 –
 		 –
 

Genomic DNA was isolated from rabbit PBMCs and subjected to PCR using HTLV-1–specific primers (670-671).

+ indicates that HTLV-1 proviral sequences were detected by PCR in inoculated rabbit PBMCs; –, that HTLV-1 proviral sequences were not detected.

*

All rabbits were negative at week 0

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