Interactions between HMRCs and human hematopoietic cells at 10 weeks after IBMT of eGFP-MSCs plus CBCD34
. | HMRC interaction with hematopoietic cells . | . | . | Hematopoietic cell interaction with HMRC . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | HMRCs interacting . | . | . | Hematopoietic cells interacting . | . | ||||
. | No. cells counted . | Total no. . | Frequency, % . | No. cells counted . | Total no. . | Frequency, % . | ||||
| CD34 | ||||||||||
| Total | 150 | 66 | 45.9 ± 13.5* | 1609 | 76 | 8.0 ± 6.2* | ||||
| Endosteal | 81 | 49 | 68.0 ± 24.8† | 1012 | 59 | 11.0 ± 9.4‡ | ||||
| Central | 59 | 17 | 14.6 ± 17.9 | 597 | 17 | 5.0 ± 3.1 | ||||
| CD15 | 100 | 3 | 1.2 ± 2.4 | 2603 | 3 | 0.14 ± 0.28 | ||||
| GlyA | 154 | 5 | 3.2 ± 4.5 | 1624 | 5 | 0.36 ± 0.49 | ||||
. | HMRC interaction with hematopoietic cells . | . | . | Hematopoietic cell interaction with HMRC . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | HMRCs interacting . | . | . | Hematopoietic cells interacting . | . | ||||
. | No. cells counted . | Total no. . | Frequency, % . | No. cells counted . | Total no. . | Frequency, % . | ||||
| CD34 | ||||||||||
| Total | 150 | 66 | 45.9 ± 13.5* | 1609 | 76 | 8.0 ± 6.2* | ||||
| Endosteal | 81 | 49 | 68.0 ± 24.8† | 1012 | 59 | 11.0 ± 9.4‡ | ||||
| Central | 59 | 17 | 14.6 ± 17.9 | 597 | 17 | 5.0 ± 3.1 | ||||
| CD15 | 100 | 3 | 1.2 ± 2.4 | 2603 | 3 | 0.14 ± 0.28 | ||||
| GlyA | 154 | 5 | 3.2 ± 4.5 | 1624 | 5 | 0.36 ± 0.49 | ||||
BM sections stained with either anti-CD34, anti-CD15, or anti–glycophorin A (GlyA) antibody were examined under fluorescent microscope (10 slides from at least 5 different mice for each cell type). The total numbers of HMRCs and immunophenotyped hematopoietic cells present in the sections were counted, and individual cells were examined for physical contact between HMRCs and hematopoietic cells. Proportions of interacting cells/slide were calculated for each cell type and expressed as the means ± SD. Interactions in CD34+ cell group were further categorized into the endosteal and the central BM groups based on the histoanatomic location of cells.
P < .005 relative to the CD15 and GlyA groups
P < .005 relative to the central group
P < .05 relative to the central group