Spatial distribution of human hematopoietic cells in the murine BM compartment at 10 weeks after IBMT of CBCD34
. | Total no. cells counted . | Total no. cells in the endosteal region . | Cells in the endosteal region/slide, % . |
|---|---|---|---|
| CD34 | 1469 | 1086 | 71.4 ± 12.5* |
| CD15 | 2940 | 451 | 14.9 ± 6.2 |
| GlyA | 1514 | 171 | 10.7 ± 2.2 |
. | Total no. cells counted . | Total no. cells in the endosteal region . | Cells in the endosteal region/slide, % . |
|---|---|---|---|
| CD34 | 1469 | 1086 | 71.4 ± 12.5* |
| CD15 | 2940 | 451 | 14.9 ± 6.2 |
| GlyA | 1514 | 171 | 10.7 ± 2.2 |
Fourteen slides from at least 8 different mice were examined to count each cell type. Slides containing 115 ± 19 cells in the diaphyseal shaft were chosen for this analysis. Because the endosteal region was arbitrarily decided within 12 cells of both endosteum, the endosteal area comprised approximately 21.4% ± 3.8% of the BM cavity in this study. The proportion of cells located in the endosteal area was calculated for each slide and expressed as the means ± SD.
P < .001 relative to the CD15 and GlyA groups