Stimulatory capacity of autologous gag mRNA-electroporated mature DCs from HIV-1–seropositive individuals
Cells and patient no. . | mDC-mock . | mDC-pv-gag . | mDC-v-gag . |
|---|---|---|---|
| PBMCs | |||
| P14 | 4 ± 2 | 236 ± 60 | 384 ± 96 |
| P15 | 4 ± 9 | 58 ± 10 | 252 ± 54 |
| P20 | 34 ± 10 | 424 ± 234 | 356 ± 56 |
| P21 | 70 ± 55 | 906 ± 112 | 290 ± 28 |
| P22 | 78 ± 18 | 116 ± 38 | 98 ± 44 |
| GM ± SE | 12 ± 9 | 225 ± 194 | 225 ± 55 |
| CD4+ T cells | |||
| P15 | 306 ± 26 | 158 ± 62 | 686 ± 18 |
| P20 | 48 ± 0 | 536 ± 0 | 328 ± 3 |
| P21 | 58 ± 0 | 280 ± 3 | 224 ± 4 |
| P22 | 36 ± 14 | 160 ± 42 | 128 ± 12 |
| GM ± SE | 74 ± 65 | 248 ± 89 | 283 ± 122 |
| CD8+ T cells | |||
| P15 | 204 ± 66 | 662 ± 72 | 908 ± 78 |
| P20 | 40 ± 8 | 1008 ± 32 | 604 ± 34 |
| P21 | 133 ± 0 | 1307 ± 3 | 1107 ± 4 |
| P22 | 130 ± 26 | 576 ± 104 | 604 ± 16 |
| GM ± SE | 109 ± 34 | 842 ± 168 | 778 ± 123 |
Cells and patient no. . | mDC-mock . | mDC-pv-gag . | mDC-v-gag . |
|---|---|---|---|
| PBMCs | |||
| P14 | 4 ± 2 | 236 ± 60 | 384 ± 96 |
| P15 | 4 ± 9 | 58 ± 10 | 252 ± 54 |
| P20 | 34 ± 10 | 424 ± 234 | 356 ± 56 |
| P21 | 70 ± 55 | 906 ± 112 | 290 ± 28 |
| P22 | 78 ± 18 | 116 ± 38 | 98 ± 44 |
| GM ± SE | 12 ± 9 | 225 ± 194 | 225 ± 55 |
| CD4+ T cells | |||
| P15 | 306 ± 26 | 158 ± 62 | 686 ± 18 |
| P20 | 48 ± 0 | 536 ± 0 | 328 ± 3 |
| P21 | 58 ± 0 | 280 ± 3 | 224 ± 4 |
| P22 | 36 ± 14 | 160 ± 42 | 128 ± 12 |
| GM ± SE | 74 ± 65 | 248 ± 89 | 283 ± 122 |
| CD8+ T cells | |||
| P15 | 204 ± 66 | 662 ± 72 | 908 ± 78 |
| P20 | 40 ± 8 | 1008 ± 32 | 604 ± 34 |
| P21 | 133 ± 0 | 1307 ± 3 | 1107 ± 4 |
| P22 | 130 ± 26 | 576 ± 104 | 604 ± 16 |
| GM ± SE | 109 ± 34 | 842 ± 168 | 778 ± 123 |
Immature DCs were electroporated with autologous proviral gag (mDC-pv-gag), viral gag (mDC-v-gag) mRNA, or mock electroporated (mDC-mock) and matured. Afterward these mDCs were cocultured with autologous PBMCs, purified CD4+ cells, or CD8+ T cells in ELISPOT plates. The amount of IFN-γ SFCs per 106 responder cells (triplicate wells) is shown as individual GM ± SE. The results from patients P15, P20, P21, and P22 are complete for all parameters. For comparative reasons only, the results of these 4 patients were summarized as overall GM ± SE.