Stimulatory capacity of codon-optimized HxB-2 HIV-1 gag mRNA-electroporated DCs from HIV-1–seropositive individuals
Patients or controls* . | PBMCs . | . | CD4+ T cells . | . | CD8+ T cells . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| . | DC-mock . | DC-h-gag . | DC-mock . | DC-h-gag . | DC-mock . | DC-h-gag . | |||
| P1 | 5 ± 3 | 561 ± 33* | 6 ± 5 | 279 ± 33 | 0 ± 0 | 428 ± 13 | |||
| P2 | 34 ± 16 | 1258 ± 48 | — | — | — | — | |||
| P3 | 12 ± 6 | 473 ± 56 | 8 ± 8 | 122 ± 26 | — | — | |||
| P4 | 438 ± 85 | 1024 ± 178 | — | — | — | — | |||
| P5 | 35 ± 12 | 707 ± 125 | 55 ± 19 | 631 ± 36 | 29 ± 2 | 1248 ± 203 | |||
| P6 | 173 ± 33 | 762 ± 69 | — | — | — | — | |||
| P7 | 28 ± 8 | 172 ± 70 | 16 ± 5 | 82 ± 9 | — | — | |||
| P8 | 86 ± 11 | 262 ± 26 | — | — | — | — | |||
| P9 | |||||||||
| iDCs | 2 ± 1 | 923 ± 67 | — | — | 2 ± 0 | 2400 ± 94 | |||
| mDCs | 11 ± 16 | 1012 ± 51 | — | — | 48 ± 23 | 2668 ± 55 | |||
| P10 | |||||||||
| iDCs | 4 ± 1 | 816 ± 162 | — | — | — | — | |||
| mDCs | 5 ± 4 | 1115 ± 205 | — | — | — | — | |||
| P11 | |||||||||
| iDCs | 6 ± 0 | 98 ± 13 | — | — | — | — | |||
| mDCs | 8 ± 7 | 398 ± 61 | — | — | 5 ± 6 | 468 ± 136 | |||
| P26 | |||||||||
| iDCs | 35 ± 1 | 994 ± 12 | — | — | — | — | |||
| mDCs | 156 ± 83 | 1465 ± 375 | — | — | — | — | |||
| P27 | |||||||||
| iDCs | 84 ± 14 | 418 ± 10 | — | — | — | — | |||
| mDCs | 88 ± 20 | 676 ± 14 | — | — | — | — | |||
| P28 | |||||||||
| iDCs | 24 ± 6 | 72 ± 16 | — | — | — | — | |||
| mDCs | 6 ± 2 | 312 ± 44 | — | — | — | — | |||
| GM ± SE | |||||||||
| iDCs | 11 ± 31 | 201 ± 100 | 14 ± 11 | 205 ± 125 | 8 ± 11 | 1086 ± 572 | |||
| mDCs | 20 ± 30 | 427 ± 237 | |||||||
| C1 | 3 ± 2 | 3 ± 3 | 39 ± 10 | 38 ± 9 | — | — | |||
| C2 | 11 ± 2 | 22 ± 9 | 6 ± 4 | 6 ± 4 | 48 ± 10 | 76 ± 14 | |||
| C3 | |||||||||
| iDCs | 37 ± 3 | 41 ± 6 | — | — | — | — | |||
| mDCs | 29 ± 5 | 30 ± 6 | — | — | — | — | |||
Patients or controls* . | PBMCs . | . | CD4+ T cells . | . | CD8+ T cells . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| . | DC-mock . | DC-h-gag . | DC-mock . | DC-h-gag . | DC-mock . | DC-h-gag . | |||
| P1 | 5 ± 3 | 561 ± 33* | 6 ± 5 | 279 ± 33 | 0 ± 0 | 428 ± 13 | |||
| P2 | 34 ± 16 | 1258 ± 48 | — | — | — | — | |||
| P3 | 12 ± 6 | 473 ± 56 | 8 ± 8 | 122 ± 26 | — | — | |||
| P4 | 438 ± 85 | 1024 ± 178 | — | — | — | — | |||
| P5 | 35 ± 12 | 707 ± 125 | 55 ± 19 | 631 ± 36 | 29 ± 2 | 1248 ± 203 | |||
| P6 | 173 ± 33 | 762 ± 69 | — | — | — | — | |||
| P7 | 28 ± 8 | 172 ± 70 | 16 ± 5 | 82 ± 9 | — | — | |||
| P8 | 86 ± 11 | 262 ± 26 | — | — | — | — | |||
| P9 | |||||||||
| iDCs | 2 ± 1 | 923 ± 67 | — | — | 2 ± 0 | 2400 ± 94 | |||
| mDCs | 11 ± 16 | 1012 ± 51 | — | — | 48 ± 23 | 2668 ± 55 | |||
| P10 | |||||||||
| iDCs | 4 ± 1 | 816 ± 162 | — | — | — | — | |||
| mDCs | 5 ± 4 | 1115 ± 205 | — | — | — | — | |||
| P11 | |||||||||
| iDCs | 6 ± 0 | 98 ± 13 | — | — | — | — | |||
| mDCs | 8 ± 7 | 398 ± 61 | — | — | 5 ± 6 | 468 ± 136 | |||
| P26 | |||||||||
| iDCs | 35 ± 1 | 994 ± 12 | — | — | — | — | |||
| mDCs | 156 ± 83 | 1465 ± 375 | — | — | — | — | |||
| P27 | |||||||||
| iDCs | 84 ± 14 | 418 ± 10 | — | — | — | — | |||
| mDCs | 88 ± 20 | 676 ± 14 | — | — | — | — | |||
| P28 | |||||||||
| iDCs | 24 ± 6 | 72 ± 16 | — | — | — | — | |||
| mDCs | 6 ± 2 | 312 ± 44 | — | — | — | — | |||
| GM ± SE | |||||||||
| iDCs | 11 ± 31 | 201 ± 100 | 14 ± 11 | 205 ± 125 | 8 ± 11 | 1086 ± 572 | |||
| mDCs | 20 ± 30 | 427 ± 237 | |||||||
| C1 | 3 ± 2 | 3 ± 3 | 39 ± 10 | 38 ± 9 | — | — | |||
| C2 | 11 ± 2 | 22 ± 9 | 6 ± 4 | 6 ± 4 | 48 ± 10 | 76 ± 14 | |||
| C3 | |||||||||
| iDCs | 37 ± 3 | 41 ± 6 | — | — | — | — | |||
| mDCs | 29 ± 5 | 30 ± 6 | — | — | — | — | |||
All samples were iDCs unless otherwise noted.
Monocyte-derived immature dendritic cells (iDCs) or monocyte-derived mature dendritic cells (mDCs) from HIV-1–seropositive patients were electroporated with hHxB-2 gag mRNA (DC-h-gag) or mock electroporated (DC-mock) and cocultured with autologous PBMCs or purified CD4+ or CD8+ T cells in ELISPOT plates for 24 hours. The resulting levels of IFN-γ SFCs per 106 responder cells (triplicate wells) are shown as geometric mean ± standard error.
— indicates not done.
The same experiment was done with dendritic cells from HIV-1–seronegative controls (C1-C3) to assay the specificity of the response. The combined results of the different seropositive donors are presented as the overall GM ± SE