Putative targets or partners of Ezh2 in HSCs
Symbol . | Name . | QTL chromosome . | Mb, if on chromosome 18 . | Correlation . |
|---|---|---|---|---|
| DNA modification, packaging, or replication | ||||
| Mcm4 | Minichromosome maintenance deficient 4 homolog (Saccharomyces cerevisiae) | 14, 18 | 20-23 | 0.7545 |
| Mcm3 | Minichromosome maintenance deficient 3 (S cerevisiae) | 9, 18 | 20-23 | 0.7284 |
| Pcna | Proliferating cell nuclear antigen | 18 | 20-23 | 0.8141 |
| Prim1 | DNA primase, p49 subunit | 18 | 12-14, 20-25 | 0.7986 |
| Hat1 | Histone aminotransferase 1 | 11 | — | 0.7973 |
| Recc1 | Replication factor C 1 | 2 | — | 0.7911 |
| Eed | Embryonic ectoderm development | 18 | 47-50 | 0.7555 |
| Pole2 | Polymerase (DNA directed), ϵ 2 (p59 subunit) | 18 | 20-23 | 0.751 |
| Mcm2 | Minichromosome maintenance deficient 2 mitotin (S cerevisiae) | 14, 18 | 20-23 | 0.739 |
| Rrm1 | Ribonucleotide reductase M1 | 4 | — | 0.7359 |
| Nap1/1 | Nucleosome assembly protein 1-like 1 | 18 | 47-50 | 0.7294 |
| Rpa3 | Replication protein A3 | 18 | 17-20, 35-38 | 0.7239 |
| H2afz | H2A histone family, member Z | 18 | 12-14 | 0.7115 |
| Setdb1 | SET domain, bifurcated 1 | 6, 17 | — | 0.7102 |
| Ahcy | S-adenosylhomocysteine hydrolase | 11 | — | 0.6855 |
| Dntt | Deoxynucleotidyltransferase, terminal | 15 | — | 0.6851 |
| Aurkb | Aurora kinase B | — | — | 0.718 |
| Nuclear organization and biogenesis, cell cycle, and cytokinesis | ||||
| Top2a | Topoisomerase (DNA) II α | 18 | 20-23 | 0.8733 |
| Smc2/1 | SMC2 structural maintenance of chromosomes 2-like 1 (yeast) | — | — | 0.8507 |
| Topbp1 | Topoisomerase (DNA) II β-binding protein | 18 | 20-25 | 0.8497 |
| Mki67 | Antigen identified by monoclonal antibody Ki 67 | 2, 18 | 20-23 | 0.837 |
| Tfdp1 | Transcription factor Dp 1 | 7 | — | 0.8299 |
| Pole2 | Polymerase (DNA directed), ϵ 2 (p59 subunit) | 18 | 20-23 | 0.7986 |
| Ccnb2 | Cyclin B2 | 13 | — | 0.7949 |
| Recc1 | Replication factor C 1 | 2 | — | 0.7911 |
| Ect2 | Ect2 oncogene | 4 | — | 0.7879 |
| Gmnn | Geminin | 18 | 14-23 | 0.7865 |
| Cdca5 | Cell division cycle associated 5 | 2 | — | 0.764 |
| Prc1 | Protein regulator of cytokinesis 1 | 5, 7 | — | 0.7584 |
| Nusap1 | Nucleolar and spindle-associated protein 1 | 14, 18 | 35-38, 71-73 | 0.7584 |
| Mcm4 | Minichromosome maintenance deficient 4 homolog (S cerevisiae) | 14, 18 | 20-23 | 0.7545 |
| Ppp1cc | Protein phosphatase 1, catalytic subunit, γ isoform | 18 | 50-53 | 0.751 |
| Nek2 | NIMA (never in mitosis gene a)-related expressed kinase 2 | 7 | — | 0.751 |
| Mad2/1 | MAD2 (mitotic arrest deficient, homolog)-like 1 (yeast) | 18 | 12-13, 35-38 | 0.7428 |
| Mcm2 | Minichromosome maintenance deficient 2 mitotin (S cerevisiae) | 14, 18 | 20-23 | 0.739 |
| Rrm1 | Ribonucleotide reductase M1 | 4 | — | 0.7359 |
| Tacc3 | Transforming, acidic coiled-coil-containing protein 3 | 18 | 12-14 | 0.7335 |
| Prim1 | DNA primase, p49 subunit | 18 | 12-14, 20-25 | 0.7321 |
| Pcna | Proliferating cell nuclear antigen | 18 | 20-23 | 0.7321 |
| Nap1/1 | Nucleosome assembly protein 1-like 1 | 18 | 47-50 | 0.7294 |
| Mcm3 | Minichromosome maintenance deficient 3 (S cerevisiae) | 9, 18 | 20-23 | 0.7284 |
| Rbl1 | Retinoblastoma-like 1 (p107) | 14 | — | 0.7268 |
| Rpa3 | Replication protein A3 | 18 | 17-20, 35-38 | 0.7239 |
| Aurkb | Aurora kinase B | — | — | 0.718 |
| H2afz | H2A histone family, member Z | 18 | 12-14 | 0.7115 |
| Setdb1 | SET domain, bifurcated 1 | 6, 17 | — | 0.7102 |
| Stk6 | Serine/threonine kinase 6 (Aurka) | 9 | — | 0.7062 |
| Dntt | Deoxynucleotidyltransferase, terminal | 15 | — | 0.6851 |
| Cdca7 | Cell division cycle-associated 7 | 9, 18 | 14-23 | 0.6843 |
| Cul2 | Cullin 2 | 18 | 50-54 | 0.682 |
| Smc4/1 | SMC4 structural maintenance of chromosomes 4-like 1 (yeast) | 3, 18 | 12-14, 25-27 | — |
| AA545217 | Expressed sequence AA545217 | 16 | — | — |
| Protein import, nuclear transport, and nucleocytoplasmic transport | ||||
| Kpna2 | Karyopherin (importin) α 2 | 18 | 51-53 | 0.78 |
| Tacc3 | Transforming, acidic coiled-coil-containing protein 3 | 18 | 12-14 | 0.7335 |
| Kpnb3 | Karyopherin (importin) β 3 | 5, 8, 13 | — | 0.7277 |
| Xpo1 | Exportin 1, CRM1 homolog (yeast) | 14 | — | 0.7581 |
Symbol . | Name . | QTL chromosome . | Mb, if on chromosome 18 . | Correlation . |
|---|---|---|---|---|
| DNA modification, packaging, or replication | ||||
| Mcm4 | Minichromosome maintenance deficient 4 homolog (Saccharomyces cerevisiae) | 14, 18 | 20-23 | 0.7545 |
| Mcm3 | Minichromosome maintenance deficient 3 (S cerevisiae) | 9, 18 | 20-23 | 0.7284 |
| Pcna | Proliferating cell nuclear antigen | 18 | 20-23 | 0.8141 |
| Prim1 | DNA primase, p49 subunit | 18 | 12-14, 20-25 | 0.7986 |
| Hat1 | Histone aminotransferase 1 | 11 | — | 0.7973 |
| Recc1 | Replication factor C 1 | 2 | — | 0.7911 |
| Eed | Embryonic ectoderm development | 18 | 47-50 | 0.7555 |
| Pole2 | Polymerase (DNA directed), ϵ 2 (p59 subunit) | 18 | 20-23 | 0.751 |
| Mcm2 | Minichromosome maintenance deficient 2 mitotin (S cerevisiae) | 14, 18 | 20-23 | 0.739 |
| Rrm1 | Ribonucleotide reductase M1 | 4 | — | 0.7359 |
| Nap1/1 | Nucleosome assembly protein 1-like 1 | 18 | 47-50 | 0.7294 |
| Rpa3 | Replication protein A3 | 18 | 17-20, 35-38 | 0.7239 |
| H2afz | H2A histone family, member Z | 18 | 12-14 | 0.7115 |
| Setdb1 | SET domain, bifurcated 1 | 6, 17 | — | 0.7102 |
| Ahcy | S-adenosylhomocysteine hydrolase | 11 | — | 0.6855 |
| Dntt | Deoxynucleotidyltransferase, terminal | 15 | — | 0.6851 |
| Aurkb | Aurora kinase B | — | — | 0.718 |
| Nuclear organization and biogenesis, cell cycle, and cytokinesis | ||||
| Top2a | Topoisomerase (DNA) II α | 18 | 20-23 | 0.8733 |
| Smc2/1 | SMC2 structural maintenance of chromosomes 2-like 1 (yeast) | — | — | 0.8507 |
| Topbp1 | Topoisomerase (DNA) II β-binding protein | 18 | 20-25 | 0.8497 |
| Mki67 | Antigen identified by monoclonal antibody Ki 67 | 2, 18 | 20-23 | 0.837 |
| Tfdp1 | Transcription factor Dp 1 | 7 | — | 0.8299 |
| Pole2 | Polymerase (DNA directed), ϵ 2 (p59 subunit) | 18 | 20-23 | 0.7986 |
| Ccnb2 | Cyclin B2 | 13 | — | 0.7949 |
| Recc1 | Replication factor C 1 | 2 | — | 0.7911 |
| Ect2 | Ect2 oncogene | 4 | — | 0.7879 |
| Gmnn | Geminin | 18 | 14-23 | 0.7865 |
| Cdca5 | Cell division cycle associated 5 | 2 | — | 0.764 |
| Prc1 | Protein regulator of cytokinesis 1 | 5, 7 | — | 0.7584 |
| Nusap1 | Nucleolar and spindle-associated protein 1 | 14, 18 | 35-38, 71-73 | 0.7584 |
| Mcm4 | Minichromosome maintenance deficient 4 homolog (S cerevisiae) | 14, 18 | 20-23 | 0.7545 |
| Ppp1cc | Protein phosphatase 1, catalytic subunit, γ isoform | 18 | 50-53 | 0.751 |
| Nek2 | NIMA (never in mitosis gene a)-related expressed kinase 2 | 7 | — | 0.751 |
| Mad2/1 | MAD2 (mitotic arrest deficient, homolog)-like 1 (yeast) | 18 | 12-13, 35-38 | 0.7428 |
| Mcm2 | Minichromosome maintenance deficient 2 mitotin (S cerevisiae) | 14, 18 | 20-23 | 0.739 |
| Rrm1 | Ribonucleotide reductase M1 | 4 | — | 0.7359 |
| Tacc3 | Transforming, acidic coiled-coil-containing protein 3 | 18 | 12-14 | 0.7335 |
| Prim1 | DNA primase, p49 subunit | 18 | 12-14, 20-25 | 0.7321 |
| Pcna | Proliferating cell nuclear antigen | 18 | 20-23 | 0.7321 |
| Nap1/1 | Nucleosome assembly protein 1-like 1 | 18 | 47-50 | 0.7294 |
| Mcm3 | Minichromosome maintenance deficient 3 (S cerevisiae) | 9, 18 | 20-23 | 0.7284 |
| Rbl1 | Retinoblastoma-like 1 (p107) | 14 | — | 0.7268 |
| Rpa3 | Replication protein A3 | 18 | 17-20, 35-38 | 0.7239 |
| Aurkb | Aurora kinase B | — | — | 0.718 |
| H2afz | H2A histone family, member Z | 18 | 12-14 | 0.7115 |
| Setdb1 | SET domain, bifurcated 1 | 6, 17 | — | 0.7102 |
| Stk6 | Serine/threonine kinase 6 (Aurka) | 9 | — | 0.7062 |
| Dntt | Deoxynucleotidyltransferase, terminal | 15 | — | 0.6851 |
| Cdca7 | Cell division cycle-associated 7 | 9, 18 | 14-23 | 0.6843 |
| Cul2 | Cullin 2 | 18 | 50-54 | 0.682 |
| Smc4/1 | SMC4 structural maintenance of chromosomes 4-like 1 (yeast) | 3, 18 | 12-14, 25-27 | — |
| AA545217 | Expressed sequence AA545217 | 16 | — | — |
| Protein import, nuclear transport, and nucleocytoplasmic transport | ||||
| Kpna2 | Karyopherin (importin) α 2 | 18 | 51-53 | 0.78 |
| Tacc3 | Transforming, acidic coiled-coil-containing protein 3 | 18 | 12-14 | 0.7335 |
| Kpnb3 | Karyopherin (importin) β 3 | 5, 8, 13 | — | 0.7277 |
| Xpo1 | Exportin 1, CRM1 homolog (yeast) | 14 | — | 0.7581 |
Ezh2 coregulated transcripts in stem cells were retrieved from www.genenetwork.org. The 100 genes of which the transcript levels correlated best with variation in Ezh2 expression were identified. These genes were classified according to their functional annotation using Webgestalt (http://genereg.oml.gov/webgestalt). Three functional categories were identified. — indicates not applicable. Correlation values refer to Pearson correlation coefficients between variation in the gene of interest and Ezh2 levels (Figure 6B-C).