Cytotoxic activity of sorted tetramer-positive lymphocytes from PBMCs of patient nos. 17, 18, and 26
. | Tetramer-positive lymphocytes sorted from PBMCs of patients . | . | . | ||
|---|---|---|---|---|---|
| Target cells . | No. 17 . | No. 18 . | No. 26 . | ||
| Nonpulsed T2 cells* | 7† | 5 | 2 | ||
| p540-pulsed T2 cells | 48 | 68 | 20 | ||
. | Tetramer-positive lymphocytes sorted from PBMCs of patients . | . | . | ||
|---|---|---|---|---|---|
| Target cells . | No. 17 . | No. 18 . | No. 26 . | ||
| Nonpulsed T2 cells* | 7† | 5 | 2 | ||
| p540-pulsed T2 cells | 48 | 68 | 20 | ||
The experiments were performed at an E/T ratio of 10:1 using 1 × 103 target cells/well
Data are expressed as percentages of lysis. Counts per minute (cpm) relative to spontaneous and maximum 51Cr releases from nonpulsed T2 cells were 353 ± 42 and 3029 ± 185; cpm relative to spontaneous and maximum 51Cr releases from nonpulsed T2 cells were 384 ± 60 and 3102 ± 178