Inhibition of CD14+ cell generation from CD34+ cells by Id2
. | – TNFα* . | . | . | + TNFα* . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | CTRL . | Id2 . | Fold changes† . | CTRL . | Id2 . | Fold changes† . | ||||
| Experiment no. 1 | 24 | 12 | 0.50 | 37 | 16 | 0.43 | ||||
| Experiment no. 2 | 11 | 7 | 0.64 | 18 | 19 | 1.06 | ||||
| Experiment no. 3 | 43 | 20 | 0.47 | 29 | 14 | 0.48 | ||||
| Experiment no. 4 | 39 | 21 | 0.54 | 23 | 19 | 0.83 | ||||
| Experiment no. 5 | 4 | 3 | 0.75 | 11 | 2 | 0.18 | ||||
| x mean | 24.2 | 12.6 | 0.58 | 23.6 | 14.0 | 0.60 | ||||
| P‡ | — | — | .01 | — | — | .059 | ||||
. | – TNFα* . | . | . | + TNFα* . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
. | CTRL . | Id2 . | Fold changes† . | CTRL . | Id2 . | Fold changes† . | ||||
| Experiment no. 1 | 24 | 12 | 0.50 | 37 | 16 | 0.43 | ||||
| Experiment no. 2 | 11 | 7 | 0.64 | 18 | 19 | 1.06 | ||||
| Experiment no. 3 | 43 | 20 | 0.47 | 29 | 14 | 0.48 | ||||
| Experiment no. 4 | 39 | 21 | 0.54 | 23 | 19 | 0.83 | ||||
| Experiment no. 5 | 4 | 3 | 0.75 | 11 | 2 | 0.18 | ||||
| x mean | 24.2 | 12.6 | 0.58 | 23.6 | 14.0 | 0.60 | ||||
| P‡ | — | — | .01 | — | — | .059 | ||||
CD34+ cells used were from 5 individual cord blood donors (experiments nos. 1-5). SEM for – TNFα fold changes, 0.05; for + TNFα, 0.15.
—indicates not done.
Cells were cultured in the presence of SCF, Flt3L, and GM-CSF with or without TNFα for 7 days after infection
Fold changes in percentages of CD14+ cells in Id2 over control (CTRL) were calculated. Control values are normalized to 1
Significance tested using a paired 2-tailed t test