Limiting dilution analysis of transplanted ALDHhiCD133+Lin- or CD133+Lin- cells
. | No. of mice engrafted/no. that received transplants (%) . | . | |
|---|---|---|---|
| No. of injected cells . | ALDHhiCD133+Lin- cells . | CD133+Lin- cells . | |
| 200 | 1/2 (50) | 0/2 (0) | |
| 500 | 1/2 (50) | 0/2 (0) | |
| 1 000 | 2/3 (67) | 0/2 (0) | |
| 5 000 | 5/5 (100) | 2/4 (50) | |
| 10 000 | 3/3 (100) | 5/6 (83) | |
| 100 000 | 5/5 (100) | 6/6 (100) | |
. | No. of mice engrafted/no. that received transplants (%) . | . | |
|---|---|---|---|
| No. of injected cells . | ALDHhiCD133+Lin- cells . | CD133+Lin- cells . | |
| 200 | 1/2 (50) | 0/2 (0) | |
| 500 | 1/2 (50) | 0/2 (0) | |
| 1 000 | 2/3 (67) | 0/2 (0) | |
| 5 000 | 5/5 (100) | 2/4 (50) | |
| 10 000 | 3/3 (100) | 5/6 (83) | |
| 100 000 | 5/5 (100) | 6/6 (100) | |
NOD/SCID β2M-null siblings (n = 42) received injections of increasing doses of ALDHhiCD133+Lin- cells or CD133+Lin- cells not purified by ALDH activity and analyzed for human engraftment 7 to 8 weeks after transplantation. SRC frequency and 95% confidence intervals were calculated using Poisson statistics at limiting dilution. ALDHhiCD133+Lin- cells was 1 in 691 cells; for CD133+Lin- cells, it was 1 in 6681 cells. The 95% confidence interval for ALDHhiCD133+Lin- cells was 246-1940 cells; for CD133+Lin- cells, it was 3073-14 525 cells.