Kinetic parameters for rFAP and APCE
. | rFAP . | . | . | APCE . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Substrate . | Km, mM . | kcat, min-1 . | kcat/Km, min-1/mM . | Km, mM . | kcat, min-1 . | kcat/Km, min-1/mM . | ||||
| Z-Gly-Pro-AMC | 0.101 ± 0.018 | 30.5 ± 3.2 | 306 ± 38 | 0.124 ± 0.024 | 32.2 ± 7.1 | 260 ± 13 | ||||
| Ala-Pro-AFC | 0.323 ± 0.052 | 64.7 ± 4.6 | 202 ± 18 | 0.272 ± 0.014 | 59.7 ± 8.1 | 218 ± 19 | ||||
| FRET peptide | 0.029 ± 0.004 | 72.2 ± 12.5 | 2491 ± 159 | 0.026 ± 0.005 | 63.4 ± 10.1 | 2445 ± 109 | ||||
. | rFAP . | . | . | APCE . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Substrate . | Km, mM . | kcat, min-1 . | kcat/Km, min-1/mM . | Km, mM . | kcat, min-1 . | kcat/Km, min-1/mM . | ||||
| Z-Gly-Pro-AMC | 0.101 ± 0.018 | 30.5 ± 3.2 | 306 ± 38 | 0.124 ± 0.024 | 32.2 ± 7.1 | 260 ± 13 | ||||
| Ala-Pro-AFC | 0.323 ± 0.052 | 64.7 ± 4.6 | 202 ± 18 | 0.272 ± 0.014 | 59.7 ± 8.1 | 218 ± 19 | ||||
| FRET peptide | 0.029 ± 0.004 | 72.2 ± 12.5 | 2491 ± 159 | 0.026 ± 0.005 | 63.4 ± 10.1 | 2445 ± 109 | ||||
Each value is the mean ± SD of 3 experiments.
FRET peptide is Arg-Lys(DABCYL)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(EDANS)-Arg.