Expression and activity analyses of the recombinant wild-type and chimeric chemokines
Chemokine/chimera . | Expression levels . | Induction of calcium flux, % . | Desensitization . |
|---|---|---|---|
| Murine MIP-1α | +++++ | 100 | Yes |
| Human RANTES | -/+ | 56 | Yes |
| First generation | |||
| MRR | +++ | 61 | Yes |
| RMM | +++ | 15 | Partial |
| MMR | + | 45 | Yes |
| RRM | ++ | 57 | Partial |
| MRM | +++ | 100 | Yes |
| RMR | + | 17 | Partial |
| Second generation | |||
| RLR | ++ | 26 | Yes |
| RTR | ++++ | 74 | Yes |
| RBR | +++ | 46 | Partial |
Chemokine/chimera . | Expression levels . | Induction of calcium flux, % . | Desensitization . |
|---|---|---|---|
| Murine MIP-1α | +++++ | 100 | Yes |
| Human RANTES | -/+ | 56 | Yes |
| First generation | |||
| MRR | +++ | 61 | Yes |
| RMM | +++ | 15 | Partial |
| MMR | + | 45 | Yes |
| RRM | ++ | 57 | Partial |
| MRM | +++ | 100 | Yes |
| RMR | + | 17 | Partial |
| Second generation | |||
| RLR | ++ | 26 | Yes |
| RTR | ++++ | 74 | Yes |
| RBR | +++ | 46 | Partial |
+ indicates just detectable; two or more “+” indicate increasing levels of detectability; and -/+ indicates sometimes detectable but sometimes not.