Primer usage in chemokine chimera generation
. | First-round PCR . | . | Second-round PCR . | . | ||
|---|---|---|---|---|---|---|
| Chimera . | Template . | Primers . | Template . | Primers . | ||
| MRR | MIP-1α | MIP-1α5′, M-CC-R | RANTES | First-round product; MIP-1α 5′, RANTES 3′ | ||
| RMM | RANTES | RANTES 5′, R-CC-M | MIP-1α | First-round product; RANTES 5′, MIP-1α 3′ | ||
| RRM | MIP-1α | MIP-1α 3′, R-C-M | RANTES | First-round product; RANTES 5′, MIP-1α 3′ | ||
| MMR | RANTES | RANTES 3′, M-C-R | MIP-1α | First-round product; MIP-1α 5′, RANTES 3′ | ||
| MRM | MRR | First-round product RRM; MIP-1α 5′, MIP-1α 3′ | — | — | ||
| RMR | RMM | First-round product MMR; RANTES 5′, RANTES 3′ | — | — | ||
| RLR | RMM | RLR, RANTES 5′ | MRR | First-round product; RANTES 5′, RANTES 3′ | ||
| RTR | RANTES | RTR, RANTES 5′ | MRR | First-round product; RANTES 5′, RANTES 3′ | ||
| RBR | RANTES | RBR, RANTES 5′ | RMR | First-round product; RANTES 5′, RANTES 3′ | ||
. | First-round PCR . | . | Second-round PCR . | . | ||
|---|---|---|---|---|---|---|
| Chimera . | Template . | Primers . | Template . | Primers . | ||
| MRR | MIP-1α | MIP-1α5′, M-CC-R | RANTES | First-round product; MIP-1α 5′, RANTES 3′ | ||
| RMM | RANTES | RANTES 5′, R-CC-M | MIP-1α | First-round product; RANTES 5′, MIP-1α 3′ | ||
| RRM | MIP-1α | MIP-1α 3′, R-C-M | RANTES | First-round product; RANTES 5′, MIP-1α 3′ | ||
| MMR | RANTES | RANTES 3′, M-C-R | MIP-1α | First-round product; MIP-1α 5′, RANTES 3′ | ||
| MRM | MRR | First-round product RRM; MIP-1α 5′, MIP-1α 3′ | — | — | ||
| RMR | RMM | First-round product MMR; RANTES 5′, RANTES 3′ | — | — | ||
| RLR | RMM | RLR, RANTES 5′ | MRR | First-round product; RANTES 5′, RANTES 3′ | ||
| RTR | RANTES | RTR, RANTES 5′ | MRR | First-round product; RANTES 5′, RANTES 3′ | ||
| RBR | RANTES | RBR, RANTES 5′ | RMR | First-round product; RANTES 5′, RANTES 3′ | ||
— indicates not applicable.