Phenotype of the lymphocyte infiltrate in small and large intestine biopsies measured by immunoenzymatic labeling
. | . | No GvHD . | . | Acute GvHD . | . | . | Chronic GvHD, cells/10 hpf's . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Cell type . | Healthy ctrls, cells/10 hpf's . | Cells/10 hpf's . | P vs healthy ctrls . | Cells/10 hpf's . | P vs healthy ctrls . | P vs no GvHD . | . | |||
| CD3+ | 130.3 ± 16.5 | 341.4 ± 70.4 | NS | 482.7 ± 56.3 | < .001 | < .01 | 501.7 ± 53.7 | |||
| CD4+ | 86.0 ± 10.2 | 208.5 ± 46.0 | NS | 199.3 ± 25.2 | < .01 | NS | 240.6 ± 33.2 | |||
| CD8+ | 44.3 ± 6.4 | 132.8 ± 26.7 | NS | 283.4 ± 33.6 | < .001 | < .001 | 260.6 ± 34.0 | |||
| FOXP3+ | 1.0 ± 0.3 | 18.6 ± 5.9 | < .001 | 5.3 ± 1.4 | < .01 | < .001 | 5.6 ± 1.5 | |||
. | . | No GvHD . | . | Acute GvHD . | . | . | Chronic GvHD, cells/10 hpf's . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Cell type . | Healthy ctrls, cells/10 hpf's . | Cells/10 hpf's . | P vs healthy ctrls . | Cells/10 hpf's . | P vs healthy ctrls . | P vs no GvHD . | . | |||
| CD3+ | 130.3 ± 16.5 | 341.4 ± 70.4 | NS | 482.7 ± 56.3 | < .001 | < .01 | 501.7 ± 53.7 | |||
| CD4+ | 86.0 ± 10.2 | 208.5 ± 46.0 | NS | 199.3 ± 25.2 | < .01 | NS | 240.6 ± 33.2 | |||
| CD8+ | 44.3 ± 6.4 | 132.8 ± 26.7 | NS | 283.4 ± 33.6 | < .001 | < .001 | 260.6 ± 34.0 | |||
| FOXP3+ | 1.0 ± 0.3 | 18.6 ± 5.9 | < .001 | 5.3 ± 1.4 | < .01 | < .001 | 5.6 ± 1.5 | |||
Number of CD3+, CD4+, CD8+, and FOXP3+ lymphocytes per 10 high-power fields (hpf's) in biopsies from healthy controls (n = 13), as well as allografted symptomatic patients without GvHD (n = 33), with acute GvHD (n = 44), and with chronic GvHD (n = 18). Results are expressed as mean ± SEM. Acute and chronic GvHD showed no significant differences.
NS indicates not significant.