Neutrophil locomotory parameters on inflamed HUVECs
. | Total migration distance, μm . | Net migration distance, μm . | Average migratory velocity, μm/s . |
|---|---|---|---|
| Control | 9.1 ± 1.4 | 7.0 ± 0.9 | 0.29 ± 0.04 |
| IC487475 | 7.2 ± 0.6 | 4.9 ± 0.8* | 0.22 ± 0.02 |
| Anti–LFA-1 | 10.8 ± 3.8 | 8.5 ± 1.5 | 0.34 ± 0.05 |
. | Total migration distance, μm . | Net migration distance, μm . | Average migratory velocity, μm/s . |
|---|---|---|---|
| Control | 9.1 ± 1.4 | 7.0 ± 0.9 | 0.29 ± 0.04 |
| IC487475 | 7.2 ± 0.6 | 4.9 ± 0.8* | 0.22 ± 0.02 |
| Anti–LFA-1 | 10.8 ± 3.8 | 8.5 ± 1.5 | 0.34 ± 0.05 |
Neutrophils were preincubated with IC487475, anti–LFA-1 (TS1/22), or vehicle and then perfused into the parallel-plate flow chamber over IL-1β–activated HUVECs at a shear stress of 2 dyne/cm2. After arrest, the geometric coordinates of neutrophil centroids were determined at 2-second intervals for 30 seconds during migration. The total migration distance is the sum of the migration step distance over 30 seconds. Net migration distance is the direct distance between the start of migration and the end of migration after 30 seconds. Average migratory velocity was determined every 2 seconds during migration and then averaged over the 30 seconds of migration. Data are presented as mean ± SEM of 10 neutrophils quantified in 4 separate experiments.
Significance compared with control (P < .05)