Change in activation-independent platelet aggregate size in the presence of normal plasma or plasma from a patient with undetectable ADAMTS13 activity
. | Control plasma . | Patient plasma . |
|---|---|---|
| Area minimum, μm2 | 45.20 ± 28.62 | 22.62 ± 0.23 |
| Area maximum, μm2 | 109.15 ± 61.71 | 652.91 ± 136.62 |
| Length minimum, μm | 11.44 ± 6.28 | 5.50 ± 0.13 |
| Length maximum, μm | 22.32 ± 9.62 | 142.65 ± 30.00 |
. | Control plasma . | Patient plasma . |
|---|---|---|
| Area minimum, μm2 | 45.20 ± 28.62 | 22.62 ± 0.23 |
| Area maximum, μm2 | 109.15 ± 61.71 | 652.91 ± 136.62 |
| Length minimum, μm | 11.44 ± 6.28 | 5.50 ± 0.13 |
| Length maximum, μm | 22.32 ± 9.62 | 142.65 ± 30.00 |
Experimental details are given in the legend to Figure 5. Washed blood cells were resuspended in plasma either from a healthy individual or from a patient with unmeasurable ADAMTS13 activity and then perfused over immobilized VWF at varying shear rates. The values represent the area of the single smallest and largest aggregate and the length of the single shortest and longest aggregate detected on the surface (mean ± SEM of 5 separate fields of view; Figure 5). The differences in maximal area (P < .007) and length (P < .005) are significant.