Change in activation-independent platelet aggregate size in the presence or absence of recombinant ADAMTS13
. | ADAMTS13 absent . | ADAMTS13 present . |
|---|---|---|
| Area minimum, μm2 | 91.96 ± 0.59 | 84.25 ± 25.41 |
| Area maximum, μm2 | 2400.00 ± 10.41 | 320.54 ± 132.04 |
| Length minimum, μm | 13.53 ± 0.91 | 20.00 ± 2.57 |
| Length maximum, μm | 238.82 ± 22.36 | 53.83 ± 14.24 |
. | ADAMTS13 absent . | ADAMTS13 present . |
|---|---|---|
| Area minimum, μm2 | 91.96 ± 0.59 | 84.25 ± 25.41 |
| Area maximum, μm2 | 2400.00 ± 10.41 | 320.54 ± 132.04 |
| Length minimum, μm | 13.53 ± 0.91 | 20.00 ± 2.57 |
| Length maximum, μm | 238.82 ± 22.36 | 53.83 ± 14.24 |
Experimental details are given in the legend to Figure 3. A suspension of washed blood cells with added soluble VWF and without or with added ADAMTS13 (protease activity added before perfusion equals 17.5% of that present in normal blood) was perfused over immobilized VWF at varying shear rates. The values (mean ± SEM of 5 different fields of view in 3 separate experiments; Figure 3) represent the area of the single smallest and largest aggregate and the length of the single shortest and longest aggregate detected on the surface. The differences in maximal area (P < .001) and length (P < .002) are significant.