Change in activation-independent platelet aggregate size in the presence or absence of EDTA
. | EDTA present . | EDTA absent . |
|---|---|---|
| Area minimum, μm2 | 23.39 ± 0.60 | 23.73 ± 0.65 |
| Area maximum, μm2 | 1187.29 ± 187.58 | 329.00 ± 43.92 |
| Length minimum, μm | 6.06 ± 0.32 | 8.64 ± 1.00 |
| Length maximum, μm | 210.35 ± 36.81 | 61.31 ± 10.55 |
. | EDTA present . | EDTA absent . |
|---|---|---|
| Area minimum, μm2 | 23.39 ± 0.60 | 23.73 ± 0.65 |
| Area maximum, μm2 | 1187.29 ± 187.58 | 329.00 ± 43.92 |
| Length minimum, μm | 6.06 ± 0.32 | 8.64 ± 1.00 |
| Length maximum, μm | 210.35 ± 36.81 | 61.31 ± 10.55 |
Experimental details are given in the legend to Figure 2. Blood containing PPACK and PG E1 was perfused over immobilized VWF at varying shear rates. The blood contained 5 mM EDTA for the initial 14 minutes, and then blood from the same donor but without EDTA was perfused for an additional 6 minutes. The values (mean ± SEM of 5 separate fields of view; Figure 2) represent the area of the single smallest and largest aggregate and the length of the single shortest and longest aggregate detected on the surface at 9 minutes (blood with EDTA) or 17 minutes (blood without EDTA) from the beginning of perfusion. The differences in maximal area (P < .001) and length (P < .001) are significant.