Inhibition of IC-21 cell adhesion to various ECM proteins by anti-αD and anti-αM antibodies
Ligand . | Anti-αD . | Anti-αM . | Anti-αD + anti-αM . |
|---|---|---|---|
| Vitronectin | 27 ± 4 | 52 ± 9 | 63 ± 5 |
| Fibrinogen | 26 ± 4 | 29 ± 9 | 48 ± 8 |
| Fibronectin | 29 ± 8 | 22 ± 1 | 45 ± 4 |
| Plasminogen | 23 ± 9 | 39 ± 5 | 56 ± 7 |
| Cyr61 | 43 ± 9 | 48 ± 8 | 59 ± 8 |
Ligand . | Anti-αD . | Anti-αM . | Anti-αD + anti-αM . |
|---|---|---|---|
| Vitronectin | 27 ± 4 | 52 ± 9 | 63 ± 5 |
| Fibrinogen | 26 ± 4 | 29 ± 9 | 48 ± 8 |
| Fibronectin | 29 ± 8 | 22 ± 1 | 45 ± 4 |
| Plasminogen | 23 ± 9 | 39 ± 5 | 56 ± 7 |
| Cyr61 | 43 ± 9 | 48 ± 8 | 59 ± 8 |
Calcein-labeled IC-21 cells were preincubated with 20 μg/mL anti-αD polyclonal antibody or 20 μg/mL anti-αM mAb M1/70 for 20 minutes at 22°C and then added to wells coated with different ligands. Adhesion was quantitated as described in “Materials and methods.” Results are expressed as a percentage of inhibition of adhesion and are means ± SE from 3 separate adhesion assays, each with triplicate measurements.