Cytokine secretion profile of the generated cells
. | Concentration, pg/mL . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| Cytokine combination (s) . | INF-γ . | IL-6 . | IL-8 . | IL-10 . | IL-12p70 . | ||||
| TPO | < 5 | < 5 | 302 ± 18 | < 5 | < 5 | ||||
| TPO + TNF-α | < 5 | 5.5 ± 0.8 | > 5000 | < 5 | < 5 | ||||
| TNF-α | < 5 | 7.0 ± 0.8 | 3152 ± 74 | < 5 | < 5 | ||||
. | Concentration, pg/mL . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| Cytokine combination (s) . | INF-γ . | IL-6 . | IL-8 . | IL-10 . | IL-12p70 . | ||||
| TPO | < 5 | < 5 | 302 ± 18 | < 5 | < 5 | ||||
| TPO + TNF-α | < 5 | 5.5 ± 0.8 | > 5000 | < 5 | < 5 | ||||
| TNF-α | < 5 | 7.0 ± 0.8 | 3152 ± 74 | < 5 | < 5 | ||||
Purified human CD34+ cells were cultured for 7 days with or without TPO and TNF-α, and the supernatants were collected. The cytokines secreted during culture were measured using a cytometric beads array system.