Table 2.

Response of aECs to proinflammatory cytokines



HUVEC1

HUVEC2
Molecule
TNF-α
IL-1β
TNF-α
IL-1β
ICAM-1, MFI   140 (7)   105 (7)   170 (10)   125 (10)  
E-selectin, %   25 (2)   34 (2)   34 (5)   36 (5)  
VCAM-1, %   22 (4)   15 (4)   25 (7)   17 (7)  
HLA-A2, MFI   27 (6)   20 (6)   44 (11)   31 (11)  
Total class I, MFI
 
198 (78)
 
189 (78)
 
237 (67)
 
162 (67)
 


HUVEC1

HUVEC2
Molecule
TNF-α
IL-1β
TNF-α
IL-1β
ICAM-1, MFI   140 (7)   105 (7)   170 (10)   125 (10)  
E-selectin, %   25 (2)   34 (2)   34 (5)   36 (5)  
VCAM-1, %   22 (4)   15 (4)   25 (7)   17 (7)  
HLA-A2, MFI   27 (6)   20 (6)   44 (11)   31 (11)  
Total class I, MFI
 
198 (78)
 
189 (78)
 
237 (67)
 
162 (67)
 

Expression of HLA class I, -A2, and adhesion molecules measured by flow cytometry was shown in mean fluorescence intensity (MFI) or the percentage of positive cells. Values in parentheses represent data obtained without cytokine treatment.

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