Table 1.

Binding of KDR peptides and controls to human HLA-A*0201

Name
Position
1 2 3 4 5 6 7 8 9 10
Score*
Fluorescence index†
KDR peptides     
   KDR6-15  6-15   L L A V A L W L C V   25   0.87  
   KDR35-43  35-43   S I Q K D I L T I   25   0.77  
   KDR82-90  82-90   G L F C K T L T I   26   0.81  
   KDR153-161  153-161   S I S N L N V S L   25   0.21  
   KDR211-219  211-219   S I M Y I V V V V   26   0.30  
   KDR227-236  227-236   V L S P S H G I E L   25   0.92  
   KDR288-297  288-297   F L S T L T I D G V   26   1.39  
   KDR505-514  505-514   A L I E G K N K T V   27   1.39  
   KDR625-634  625-634   L I M E L K N A S L   27   0.77  
   KDR766-774  766-774   I I L V G T A V I   26   0.82  
   KDR775-783  775-783   A M F F W L L L V   25   0.74  
   KDR781-789  781-789   L L V I I L R T V   28   0.85  
   KDR881-889  881-889   A L M S E L K I L   26   1.01  
   KDR888-896  888-896   I L I H I G H H L   25   0.57  
   KDR1034-1044  1035-1044   L L S E K N V V K I   30   0.87  
   KDR1076-1084  1076-1084   T I F D R V Y T I   25   0.59  
   KDR1093-1101  1093-1101   V L L W E I F S L   29   1.03  
   KDR1263-1272  1263-1272   V L A S E E L K T L   28   1.04  
   KDR1342-1351  1342-1351   I L Q P D S G T T L   26   0.62  
Control peptides     
   Melan-A27-35  27-35   A A G I G I L T V   25   0.84  
   TRP2288-296  288-296   S L D D Y N H L V   25   0.81  
   TYR454-463‡
 		 454-463
 		 D S D P D S F Q D Y
 		 1
 		 0.01
 
Name
Position
1 2 3 4 5 6 7 8 9 10
Score*
Fluorescence index†
KDR peptides     
   KDR6-15  6-15   L L A V A L W L C V   25   0.87  
   KDR35-43  35-43   S I Q K D I L T I   25   0.77  
   KDR82-90  82-90   G L F C K T L T I   26   0.81  
   KDR153-161  153-161   S I S N L N V S L   25   0.21  
   KDR211-219  211-219   S I M Y I V V V V   26   0.30  
   KDR227-236  227-236   V L S P S H G I E L   25   0.92  
   KDR288-297  288-297   F L S T L T I D G V   26   1.39  
   KDR505-514  505-514   A L I E G K N K T V   27   1.39  
   KDR625-634  625-634   L I M E L K N A S L   27   0.77  
   KDR766-774  766-774   I I L V G T A V I   26   0.82  
   KDR775-783  775-783   A M F F W L L L V   25   0.74  
   KDR781-789  781-789   L L V I I L R T V   28   0.85  
   KDR881-889  881-889   A L M S E L K I L   26   1.01  
   KDR888-896  888-896   I L I H I G H H L   25   0.57  
   KDR1034-1044  1035-1044   L L S E K N V V K I   30   0.87  
   KDR1076-1084  1076-1084   T I F D R V Y T I   25   0.59  
   KDR1093-1101  1093-1101   V L L W E I F S L   29   1.03  
   KDR1263-1272  1263-1272   V L A S E E L K T L   28   1.04  
   KDR1342-1351  1342-1351   I L Q P D S G T T L   26   0.62  
Control peptides     
   Melan-A27-35  27-35   A A G I G I L T V   25   0.84  
   TRP2288-296  288-296   S L D D Y N H L V   25   0.81  
   TYR454-463‡
 		 454-463
 		 D S D P D S F Q D Y
 		 1
 		 0.01
 
*

Algorithmic score of the probability of being processed and presented according to SYFPEITHI software (www.syfpeithi.de)

†

(Mean fluorescence with peptide–mean fluorescence without peptide)/(mean fluorescence without peptide). Shown are representative results of 2 independent experiments

‡

Peptide sequence derived from tyrosinase, known to bind to HLA-A1 (negative controls)

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