Summary of primers used for real-time RT-PCR in this study
. | Primer sequences . | . | . | . | |
|---|---|---|---|---|---|
| Gene . | Forward . | Reverse . | Size, bp . | Annealing temperature, °C . | |
| 18S | 5′-GATGCGGGGCGTTATT-3′ | 5′-TGAGGTTTCCCGTGTTGTCA-3′ | 167 | 59 | |
| GATA1 | 5′-TGGAGACTTTGAAGACAGAGCGGCTGAG-3′ | 5′-GAAGCTTGGGAGAGGAATAGGCTGCTGA-3′ | 174 | 63 | |
| BST2 | 5′-CCTGCTCGGCTTTTCGCTTGAACAT-3′ | 5′-CGGAGGGAGGCTCTGGAGGGAGAC-3′ | 199 | 62 | |
| KRT18 | 5′-GAGTATGAGGCCCTGCTGAACATCA-3′ | 5′-GCGGGTGGTGGTCTTTTGGAT-3′ | 150 | 62 | |
| DUSP6 | 5′-GCTGTGCCAAAGACTCCACCAACTT-3′ | 5′-GGCCTCAGGGAAAAACTGGGACA-3′ | 176 | 62 | |
| BCL2 | 5′-CTGCACCTGACGCCCTTCACC-3′ | 5′-CACATGACCCCACCGAACTCAAAGA-3′ | 119 | 61 | |
| CD36 | 5′-ATTGGTGCTGTCCTGGCTGTGTTT-3′ | 5′-GTTCATCATCACTTCCTGTGGATTTTGC-3′ | 195 | 59 | |
| RHAG | 5′-GGCATGAAAATGAAGAGTCCGCATAC-3′ | 5′-CTGGAGAAGGCAAAGGCTGTGA-3′ | 187 | 61 | |
| MDR1 | 5′-CAGGAACCTGTATTGTTTGCCACCAC-3′ | 5′-TGCTTCTGCCCACCACTCAACTG-3′ | 188 | 59 | |
. | Primer sequences . | . | . | . | |
|---|---|---|---|---|---|
| Gene . | Forward . | Reverse . | Size, bp . | Annealing temperature, °C . | |
| 18S | 5′-GATGCGGGGCGTTATT-3′ | 5′-TGAGGTTTCCCGTGTTGTCA-3′ | 167 | 59 | |
| GATA1 | 5′-TGGAGACTTTGAAGACAGAGCGGCTGAG-3′ | 5′-GAAGCTTGGGAGAGGAATAGGCTGCTGA-3′ | 174 | 63 | |
| BST2 | 5′-CCTGCTCGGCTTTTCGCTTGAACAT-3′ | 5′-CGGAGGGAGGCTCTGGAGGGAGAC-3′ | 199 | 62 | |
| KRT18 | 5′-GAGTATGAGGCCCTGCTGAACATCA-3′ | 5′-GCGGGTGGTGGTCTTTTGGAT-3′ | 150 | 62 | |
| DUSP6 | 5′-GCTGTGCCAAAGACTCCACCAACTT-3′ | 5′-GGCCTCAGGGAAAAACTGGGACA-3′ | 176 | 62 | |
| BCL2 | 5′-CTGCACCTGACGCCCTTCACC-3′ | 5′-CACATGACCCCACCGAACTCAAAGA-3′ | 119 | 61 | |
| CD36 | 5′-ATTGGTGCTGTCCTGGCTGTGTTT-3′ | 5′-GTTCATCATCACTTCCTGTGGATTTTGC-3′ | 195 | 59 | |
| RHAG | 5′-GGCATGAAAATGAAGAGTCCGCATAC-3′ | 5′-CTGGAGAAGGCAAAGGCTGTGA-3′ | 187 | 61 | |
| MDR1 | 5′-CAGGAACCTGTATTGTTTGCCACCAC-3′ | 5′-TGCTTCTGCCCACCACTCAACTG-3′ | 188 | 59 | |