Primers and probe used in Q-PCR for PRDM1 copy number determination
. | 5′ Primer . | 3′ Primer . | Probe . |
|---|---|---|---|
| PRDM1 Ex 3 | ATACCAAAGGGCACACGTT | CCAGCTCTTTTAGCTCCATTT | FAM-CACAGTTCCTAAGAACGCCAACAGGT-TAMRA |
| PRDM1 Ex 4 | ATGTGAATCCAGCACACTCTC | CCGACAATACCACACAAGAAG | FAM-CAAAACCTGGCTGCGTGTCAGA-TAMRA |
| PRDM1 Ex 5 | AGCACAAACACAGAGCAGTCTA | TTTAGGATTTCTTTCACGCTGT | FAM-TGCCCAAAGAATGTCCCAAAGAGA-TAMRA |
| PRDM1 Ex 7 | CCAGTGCCACAAGAACTACATC | CACTGATGTCAAACTTCTCGATT | FAM-CCCTTGGAAGATCTGACCCGAATC-TAMRA |
| SFRS | TTAGGATGAGCCTCTCCTAGACTT | TCCTACAGCCACTTCGAGAAC | FAM-CCCAAGGCCCACTTGGTCCAT-TAMRA |
. | 5′ Primer . | 3′ Primer . | Probe . |
|---|---|---|---|
| PRDM1 Ex 3 | ATACCAAAGGGCACACGTT | CCAGCTCTTTTAGCTCCATTT | FAM-CACAGTTCCTAAGAACGCCAACAGGT-TAMRA |
| PRDM1 Ex 4 | ATGTGAATCCAGCACACTCTC | CCGACAATACCACACAAGAAG | FAM-CAAAACCTGGCTGCGTGTCAGA-TAMRA |
| PRDM1 Ex 5 | AGCACAAACACAGAGCAGTCTA | TTTAGGATTTCTTTCACGCTGT | FAM-TGCCCAAAGAATGTCCCAAAGAGA-TAMRA |
| PRDM1 Ex 7 | CCAGTGCCACAAGAACTACATC | CACTGATGTCAAACTTCTCGATT | FAM-CCCTTGGAAGATCTGACCCGAATC-TAMRA |
| SFRS | TTAGGATGAGCCTCTCCTAGACTT | TCCTACAGCCACTTCGAGAAC | FAM-CCCAAGGCCCACTTGGTCCAT-TAMRA |