Table 1.

Summary of oligonucleotide primers

Primer name
Position
Sequence
Experimental use
PRDM1.E2.Probe.F   PRDM1 intron 1 nt 1490-1515   AGCACTCTGCATCTGCTTTTAAAGTC   Probe generation for Southern blot (Figure 1B)  
PRDM1.E2.Probe.R   PRDM1 intron 2 nt 74-54   ACAAGGCCTCAAGGGCAAGTA   
c-myb.Int1.ProbeF   c-myb intron 1 nt 1144-64   GAGGCAAAGCAAATCCATTCG   Probe generation for Southern blot (Figure 1B)  
c-myb.Int1.ProbeR   c-myb intron 1 nt 1450-28   CCTGTGAAGTCCTAAAGGCGAAA   
PRDM1.E2.75.F   PRDM1 exon 2 nt 75-95   GGACATGGAGGATGCGGATAT   Analysis of PRDM1α expression (Figure 1C) and splicing aberration (Figure 3)  
PRDM1.E4.60.R   PRDM1 exon 4 nt 60-36   GTTGCTTTTCTCTTCATTAAAGCCG   
PRDM1.altE1.F   PRDM1 exon 1β 16-37   TTTGCCATTCACTGCAGTAGCA   PRDM1β expression (Figure 1C)  
PRDM1.E4.60.R   PRDM1 exon 4 nt 60-36   GTTGCTTTTCTCTTCATTAAAGCCG   
c-myb.E1.195.F/   c-myb exon 1 nt 195-212   GCGCCATGGCCCGAAGAC   Analysis of c-myb expression from the regular promoter and alternative (beta) promoter (Figure 2A)  
c-myb.altE1.98.F   c-myb exon 1β 7-27   CAGGGGAAGAGAAACCCGACT   
c-myb.E2.98.R   c-myb exon 2 nt 98-78   TTTTCCCCAAGTGACGCTTTC   
PRDM1.E2.75.F   PRDM1 exon 2 nt 75-95   GGACATGGAGGATGCGGATAT   Forward primer for 3′ RACE (Figure 1C)  
PRDM1.E2.126.F   PRDM1 exon 2 nt 126-95   GAAGTGTACATACATTGTGAACGACCA   Forward nested primer for 3′ RACE (Figure 1C)  
c-myb.E2.98.R   c-myb exon 2 nt 98-78   TTTTCCCCAAGTGACGCTTTC   Reverse primer for 5′ RACE (Figure 2)  
c-myb.E2.38.R   c-myb exon 2 nt 38-15   TCTCAAAGTCCTCATCATCCTCGT   Reverse nested primer for 5′ RACE (Figure 2)  
PRDM1.E2.1.F   PRDM1 exon 2 nt 1-23   GCTGCCCCCAAGTGTAACTCCAG   cDNA generation for ORF sequencing  
PRDM1.E4.60.R   PRDM1 exon 4 nt 60-36   GTTGCTTTTCTCTTCATTAAAGCCG   
PRDM1.E4.52.F   PRDM1 exon 4 nt 52-73   AAAAGCAACTGGATGCGCTATG   cDNA generation for ORF sequencing  
PRDM1.E5.477.R   PRDM1 exon 5 nt 477-456   AGGACGCGTTCAAGTAAGCGTA   
PRDM1.E5.454.F   PRDM1 exon 5 nt 454-475   CCTACGCTTACTTGAACGCGTC   cDNA generation for ORF sequencing  
PRDM1.E5.1077.R/   PRDM1 exon 5 nt 1077-1057   CGCAAACGTTGCATTCGTACT   
PRDM1.E7.67.R   PRDM1 exon 7 nt 67-47   TCTCTCCAGAATGGAGTCGCA   
PRDM1.E5.1057.F   PRDM1 exon 5 nt 1057-1077   AGTACGAATGCAACGTTTGCG   cDNA generation for ORF sequencing and analysis of splicing aberration (Figure 3A)  
PRDM1.E7.290.R   PRDM1 exon 7 nt 290-268   TCGATTTCTTCATTGATTCGGGT   
PRDM1.E7.32.F/   PRDM1 exon 7 nt 32-52   ATCTCAAGACCCACCTGCGAC   cDNA generation for ORF sequencing  
PRDM1.E7.257.F   PRDM1 exon 7 nt 257-278   TGGAAGATCTGACCCGAATCAA   
PRDM1.E7.585.R   PRDM1 exon 7 nt 585-559   CTGAAAATCTTAAGGATCCATTGGTTC   
PRDM1.E3.79.F   PRDM1 exon 3 nt 79-102   GACACAGTTCCTAAGAACGCCAAC   cDNA generation for ORF sequencing  
PRDM1.E5.46.R   PRDM1 exon 5 nt 46-24   TCATTTTTCTCAGTGCTCGGTTG   
PRDM1.E5.170.F   PRDM1 exon 5 nt 170-194   GGACCTCGATGACTTTAGAAGACGT   cDNA generation for ORF sequencing  
PRDM1.E5.575.R   PRDM1 exon 5 nt 575-552   GAACTTGGGGTAGTGAGCGTTGTA   
PRDM1.E2.157.F   PRDM1 exon 2 nt 157-175   TGGGATTCTGGTGCTGATG   cDNA generation for exon 2 frameshift (Figure 5)  
PRDM1.E2.245.R   PRDM1 exon 2 nt 245-225   TCACTGTTGGTGGCATACTTG   
PRDM1.5BR.F1   PRDM1 exon 2 121-150   GAAGAGAAGTGTACATACATTGTGAACGAC   Forward primers for cloning of 5′ breakpoint junction by LA-PCR (Figure 1B)  
PRDM1.5BR.F2   PRDM1 exon 2 nt 212-335   GGAATCTGCTTTTCAAGTATGCCA   
PRDM1.3BR.F   PRDM1 intron 2 nt 666-641   CCCCTTTGAAACAACAAAGAGTGTAC   Cloning of 3′ breakpoint junction (Figure 1B)  
PRDM1.3BR.R
 		 c-myb intron 1 nt 1181-1158
 		 AACAATCTAAGGAAAGGCGAATGG
 
 
Primer name
Position
Sequence
Experimental use
PRDM1.E2.Probe.F   PRDM1 intron 1 nt 1490-1515   AGCACTCTGCATCTGCTTTTAAAGTC   Probe generation for Southern blot (Figure 1B)  
PRDM1.E2.Probe.R   PRDM1 intron 2 nt 74-54   ACAAGGCCTCAAGGGCAAGTA   
c-myb.Int1.ProbeF   c-myb intron 1 nt 1144-64   GAGGCAAAGCAAATCCATTCG   Probe generation for Southern blot (Figure 1B)  
c-myb.Int1.ProbeR   c-myb intron 1 nt 1450-28   CCTGTGAAGTCCTAAAGGCGAAA   
PRDM1.E2.75.F   PRDM1 exon 2 nt 75-95   GGACATGGAGGATGCGGATAT   Analysis of PRDM1α expression (Figure 1C) and splicing aberration (Figure 3)  
PRDM1.E4.60.R   PRDM1 exon 4 nt 60-36   GTTGCTTTTCTCTTCATTAAAGCCG   
PRDM1.altE1.F   PRDM1 exon 1β 16-37   TTTGCCATTCACTGCAGTAGCA   PRDM1β expression (Figure 1C)  
PRDM1.E4.60.R   PRDM1 exon 4 nt 60-36   GTTGCTTTTCTCTTCATTAAAGCCG   
c-myb.E1.195.F/   c-myb exon 1 nt 195-212   GCGCCATGGCCCGAAGAC   Analysis of c-myb expression from the regular promoter and alternative (beta) promoter (Figure 2A)  
c-myb.altE1.98.F   c-myb exon 1β 7-27   CAGGGGAAGAGAAACCCGACT   
c-myb.E2.98.R   c-myb exon 2 nt 98-78   TTTTCCCCAAGTGACGCTTTC   
PRDM1.E2.75.F   PRDM1 exon 2 nt 75-95   GGACATGGAGGATGCGGATAT   Forward primer for 3′ RACE (Figure 1C)  
PRDM1.E2.126.F   PRDM1 exon 2 nt 126-95   GAAGTGTACATACATTGTGAACGACCA   Forward nested primer for 3′ RACE (Figure 1C)  
c-myb.E2.98.R   c-myb exon 2 nt 98-78   TTTTCCCCAAGTGACGCTTTC   Reverse primer for 5′ RACE (Figure 2)  
c-myb.E2.38.R   c-myb exon 2 nt 38-15   TCTCAAAGTCCTCATCATCCTCGT   Reverse nested primer for 5′ RACE (Figure 2)  
PRDM1.E2.1.F   PRDM1 exon 2 nt 1-23   GCTGCCCCCAAGTGTAACTCCAG   cDNA generation for ORF sequencing  
PRDM1.E4.60.R   PRDM1 exon 4 nt 60-36   GTTGCTTTTCTCTTCATTAAAGCCG   
PRDM1.E4.52.F   PRDM1 exon 4 nt 52-73   AAAAGCAACTGGATGCGCTATG   cDNA generation for ORF sequencing  
PRDM1.E5.477.R   PRDM1 exon 5 nt 477-456   AGGACGCGTTCAAGTAAGCGTA   
PRDM1.E5.454.F   PRDM1 exon 5 nt 454-475   CCTACGCTTACTTGAACGCGTC   cDNA generation for ORF sequencing  
PRDM1.E5.1077.R/   PRDM1 exon 5 nt 1077-1057   CGCAAACGTTGCATTCGTACT   
PRDM1.E7.67.R   PRDM1 exon 7 nt 67-47   TCTCTCCAGAATGGAGTCGCA   
PRDM1.E5.1057.F   PRDM1 exon 5 nt 1057-1077   AGTACGAATGCAACGTTTGCG   cDNA generation for ORF sequencing and analysis of splicing aberration (Figure 3A)  
PRDM1.E7.290.R   PRDM1 exon 7 nt 290-268   TCGATTTCTTCATTGATTCGGGT   
PRDM1.E7.32.F/   PRDM1 exon 7 nt 32-52   ATCTCAAGACCCACCTGCGAC   cDNA generation for ORF sequencing  
PRDM1.E7.257.F   PRDM1 exon 7 nt 257-278   TGGAAGATCTGACCCGAATCAA   
PRDM1.E7.585.R   PRDM1 exon 7 nt 585-559   CTGAAAATCTTAAGGATCCATTGGTTC   
PRDM1.E3.79.F   PRDM1 exon 3 nt 79-102   GACACAGTTCCTAAGAACGCCAAC   cDNA generation for ORF sequencing  
PRDM1.E5.46.R   PRDM1 exon 5 nt 46-24   TCATTTTTCTCAGTGCTCGGTTG   
PRDM1.E5.170.F   PRDM1 exon 5 nt 170-194   GGACCTCGATGACTTTAGAAGACGT   cDNA generation for ORF sequencing  
PRDM1.E5.575.R   PRDM1 exon 5 nt 575-552   GAACTTGGGGTAGTGAGCGTTGTA   
PRDM1.E2.157.F   PRDM1 exon 2 nt 157-175   TGGGATTCTGGTGCTGATG   cDNA generation for exon 2 frameshift (Figure 5)  
PRDM1.E2.245.R   PRDM1 exon 2 nt 245-225   TCACTGTTGGTGGCATACTTG   
PRDM1.5BR.F1   PRDM1 exon 2 121-150   GAAGAGAAGTGTACATACATTGTGAACGAC   Forward primers for cloning of 5′ breakpoint junction by LA-PCR (Figure 1B)  
PRDM1.5BR.F2   PRDM1 exon 2 nt 212-335   GGAATCTGCTTTTCAAGTATGCCA   
PRDM1.3BR.F   PRDM1 intron 2 nt 666-641   CCCCTTTGAAACAACAAAGAGTGTAC   Cloning of 3′ breakpoint junction (Figure 1B)  
PRDM1.3BR.R
 		 c-myb intron 1 nt 1181-1158
 		 AACAATCTAAGGAAAGGCGAATGG
 
 
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