Primer and probe sequences used for JAK2V617F and clonality assays
Primer . | Probe sequence . |
|---|---|
| HUMARA-Primer-F | GCTGTGAAGGTTGCTGTTCCTCAT |
| HUMARA-Primer-R | TCCAGAATCTGTTCCAGAGCGTGC |
| JAK2-PCR-Primer-F | AAGCTTTCTCACAAGCATTTGGTTT |
| JAK2-PCR-Primer-R | AGAAAGGCATTAGAAAGCCTGTAGTT |
| JAK2-Probe-WT; VIC | TCTCCACAGACACATAC |
| JAK2-Probe-V617F; FAM | TCCACAGAAACATAC |
Primer . | Probe sequence . |
|---|---|
| HUMARA-Primer-F | GCTGTGAAGGTTGCTGTTCCTCAT |
| HUMARA-Primer-R | TCCAGAATCTGTTCCAGAGCGTGC |
| JAK2-PCR-Primer-F | AAGCTTTCTCACAAGCATTTGGTTT |
| JAK2-PCR-Primer-R | AGAAAGGCATTAGAAAGCCTGTAGTT |
| JAK2-Probe-WT; VIC | TCTCCACAGACACATAC |
| JAK2-Probe-V617F; FAM | TCCACAGAAACATAC |