Molecular studies and immunohistochemistry in cutaneous samples from patients 1, 2, and 3
. | Molecular studies . | . | . | Immunohistochemistry . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | Epidermis, no. positive cells/mm . | . | . | . | Dermis, ratio category . | . | . | ||||||||
| Patient . | Immunoscopy: Vβ family of T-cell clones . | CD158k* . | CD158e† . | CD3 . | CD4 . | CD8 . | CD158k‡ . | CD4/CD3 . | CD8/CD3 . | CD158k‡/CD3 . | ||||||||
| 1 | 17, 6, 16 | + | - | 68 | 59 | 2 | 2 | 4 | 1 | 1 | ||||||||
| 1§ | 17, 15, 16 | + | - | 54 | 44 | 12 | 9 | 4 | 2 | 2 | ||||||||
| 2 | 2 | + | - | 6 | 3 | 0 | 3 | 4 | 0 | 2 | ||||||||
| 3 | 8 | + | - | 32 | 30 | 0 | 14 | 4 | 0 | 3 | ||||||||
. | Molecular studies . | . | . | Immunohistochemistry . | . | . | . | . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | Epidermis, no. positive cells/mm . | . | . | . | Dermis, ratio category . | . | . | ||||||||
| Patient . | Immunoscopy: Vβ family of T-cell clones . | CD158k* . | CD158e† . | CD3 . | CD4 . | CD8 . | CD158k‡ . | CD4/CD3 . | CD8/CD3 . | CD158k‡/CD3 . | ||||||||
| 1 | 17, 6, 16 | + | - | 68 | 59 | 2 | 2 | 4 | 1 | 1 | ||||||||
| 1§ | 17, 15, 16 | + | - | 54 | 44 | 12 | 9 | 4 | 2 | 2 | ||||||||
| 2 | 2 | + | - | 6 | 3 | 0 | 3 | 4 | 0 | 2 | ||||||||
| 3 | 8 | + | - | 32 | 30 | 0 | 14 | 4 | 0 | 3 | ||||||||
With the use of immunohistochemistry, the proportion of labeled cells was evaluated separately in the epidermis and in the dermis. In the epidermis, the numbers of CD3+, CD4+, CD8+, and AZ158+ lymphocytes per millimeter were evaluated on the total epidermal length. In the dermis, semiquantitative analysis was performed in which CD4/CD3, CD8/CD3, and AZ158/CD3 ratios were categorized into 4 groups: group 1 (0%-25%), group 2 (greater than 25%-50%), group 3 (greater than 50%-75%), and group 4 (greater 75%-100%).
Molecular study of CD158k in the skin consisted of RT-PCR with consensus primers applied to total RNA extracted from the skin samples used for immunohistochemistry.
Molecular study of CD158e in the skin consisted of RT-PCR with consensus primers applied to total RNA extracted from the skin samples used for immunohistochemistry.
Immunohistochemistry analysis for the CD158k+ population was performed using the AZ158 mAb, which recognizes KIR3DL1/CD158e and KIR3DL2/CD158k.
Data after 1 year of disease progression.