Table 1. Human and porcine fVIII... | American Society of Hematology

Table 1.

Human and porcine fVIII expression from genetically modified MSCs

Transgene	DNA copy no., fVIII transgenes per diploid genome equivalent	FVIII transcripts per cell^*	FVIII activity, milliunits/24 h†
Human fVIII	0.15‡	140	14
Porcine fVIII	0.18§	116	84

Transgene	DNA copy no., fVIII transgenes per diploid genome equivalent	FVIII transcripts per cell^*	FVIII activity, milliunits/24 h†
Human fVIII	0.15‡	140	14
Porcine fVIII	0.18§	116	84

MSCs were transduced with MSCV-human fVIII or MSCV-porcine fVIII at equal MOI and analyzed for gene marking, viral RNA levels, and fVIII production by qPCR, qRT-PCR, and chromogenic fVIII activity assay.

n = 3.

†

n = 6.

‡

n = 2.

n = 3.

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