Primers and probes for genotyping of interbred mice with combined deficiencies of Ldlr, Apobec1, and Fg
Gene primer identification* . | Sequence of oligonucleotide . |
|---|---|
| LDLR | |
| WT-LDLR-F | 5′-CAAGACGTGCTCCCAGGATGACTTC |
| WT/N-LDLR-R (common)* | 5′-CTTGTCCTTGCAGTCTGCCTCGCC |
| WT-LDLR-FITC† | 5′-CAATCTCGGTCTCCATCACACAC-FITC |
| WT-LDLR-R640‡ | 5′-R640-ACTGCGGGGAGATGCACTTGCCATC-P |
| Null-LDLR-NEO | 5′-GATTGGGAAGACAATAGCAGGCATGC |
| Null-LDR-FITC† | 5′-GCTGGTTCTTTCCGCCTCAGAA-FITC |
| Null-LDLR-R705‡ | 5′-R705-CATAGAGCCCACCGCATCCCCA-P |
| APOBEC1 | |
| WT-APOBEC1-F | 5′-GCCACTATGCCCAGGTCA |
| WT/N-APOBEC1-R (common)* | 5′-CTCCAATACATACAGTTTCACCCAC |
| WT-APOBEC1-FITC† | 5′-TTAGAGTATTGTTACTGCTGGAGGAA-FITC |
| WT-APOBEC1-R640‡ | 5′-R640-TCGTCAACTACCCCCCTCTTCAAACG-P |
| Null-APOBEC1-NEO | 5′-ACAAGCAAAACCAAATTAAGGGCCA |
| Null-APOBEC1-FITC† | 5′-TGCTGATCTCGTTCTTCAGGCTAT-FITC |
| Null-APOBEC1-R705‡ | 5′-R705-AACTGACACATTTGGAAACCACAG TACTTAGAACCAC-P |
| FG | |
| WT/N-FG-F (common)* | 5′-CACAGCGGCTTGTCATTAG |
| WT-FG-R | 5′-CTGAAAGACCTGTCTTTGC |
| WT-FG-FITC† | 5′-TGAGCCACCCTTAGAAATACAGACC-FITC |
| WT-FG-R640‡ | 5′-R640-AGACATGAAAGGAGGAAAAGTTCCC-P |
| Null-FG-NEO | 5′-ACAAGCAAAACCAAATTAAGGGCCA |
| Null-FG-FITC† | 5′-TGCTGATCTCGTTCTTCAGGCTAT-FITC |
| Null-FG-R705‡ | 5′-R705-AACTGACACATTTGGAAACCACAGT ACTTAGAACCAC-P |
Gene primer identification* . | Sequence of oligonucleotide . |
|---|---|
| LDLR | |
| WT-LDLR-F | 5′-CAAGACGTGCTCCCAGGATGACTTC |
| WT/N-LDLR-R (common)* | 5′-CTTGTCCTTGCAGTCTGCCTCGCC |
| WT-LDLR-FITC† | 5′-CAATCTCGGTCTCCATCACACAC-FITC |
| WT-LDLR-R640‡ | 5′-R640-ACTGCGGGGAGATGCACTTGCCATC-P |
| Null-LDLR-NEO | 5′-GATTGGGAAGACAATAGCAGGCATGC |
| Null-LDR-FITC† | 5′-GCTGGTTCTTTCCGCCTCAGAA-FITC |
| Null-LDLR-R705‡ | 5′-R705-CATAGAGCCCACCGCATCCCCA-P |
| APOBEC1 | |
| WT-APOBEC1-F | 5′-GCCACTATGCCCAGGTCA |
| WT/N-APOBEC1-R (common)* | 5′-CTCCAATACATACAGTTTCACCCAC |
| WT-APOBEC1-FITC† | 5′-TTAGAGTATTGTTACTGCTGGAGGAA-FITC |
| WT-APOBEC1-R640‡ | 5′-R640-TCGTCAACTACCCCCCTCTTCAAACG-P |
| Null-APOBEC1-NEO | 5′-ACAAGCAAAACCAAATTAAGGGCCA |
| Null-APOBEC1-FITC† | 5′-TGCTGATCTCGTTCTTCAGGCTAT-FITC |
| Null-APOBEC1-R705‡ | 5′-R705-AACTGACACATTTGGAAACCACAG TACTTAGAACCAC-P |
| FG | |
| WT/N-FG-F (common)* | 5′-CACAGCGGCTTGTCATTAG |
| WT-FG-R | 5′-CTGAAAGACCTGTCTTTGC |
| WT-FG-FITC† | 5′-TGAGCCACCCTTAGAAATACAGACC-FITC |
| WT-FG-R640‡ | 5′-R640-AGACATGAAAGGAGGAAAAGTTCCC-P |
| Null-FG-NEO | 5′-ACAAGCAAAACCAAATTAAGGGCCA |
| Null-FG-FITC† | 5′-TGCTGATCTCGTTCTTCAGGCTAT-FITC |
| Null-FG-R705‡ | 5′-R705-AACTGACACATTTGGAAACCACAGT ACTTAGAACCAC-P |