Table 2. Time-dependent... | American Society of Hematology

Table 2.

Time-dependent glucocorticoid-mediated dissociation of GR chaperon proteins

	Densitometric analysis, arbitrary units
Time and treatment	HSP70	HSP90	FKBP51	FKBP52
0.5 h
Control	119 ± 12	104 ± 9	139 ± 16	94 ± 35
Dex	124 ± 9	127 ± 9^*	144 ± 20	130 ± 32
Pred	156 ± 9^*	136 ± 8^*	112 ± 15	131 ± 34
2 h
Control	103 ± 9	76 ± 7	142 ± 15	160 ± 11
Dex	108 ± 29	144 ± 4^*	114 ± 18^*	140 ± 32^*
Pred	76 ± 10^*	130 ± 16^*	102 ± 9^*	161 ± 20^*

	Densitometric analysis, arbitrary units
Time and treatment	HSP70	HSP90	FKBP51	FKBP52
0.5 h
Control	119 ± 12	104 ± 9	139 ± 16	94 ± 35
Dex	124 ± 9	127 ± 9^*	144 ± 20	130 ± 32
Pred	156 ± 9^*	136 ± 8^*	112 ± 15	131 ± 34
2 h
Control	103 ± 9	76 ± 7	142 ± 15	160 ± 11
Dex	108 ± 29	144 ± 4^*	114 ± 18^*	140 ± 32^*
Pred	76 ± 10^*	130 ± 16^*	102 ± 9^*	161 ± 20^*

PRP was incubated with vehicle (control), 10 μM dexamethasone (Dex), or 10 μM prednisolone (Pred) for the time indicated. Then, immunoprecipitation with specific antibodies to HSP70, HSP90, FKBP51, or FKBP52 was conducted as described in “Materials and methods,” and WB analysis for GR was applied to reveal direct protein association. See Figure 7 for an example of basal detection of GR association with each single chaperone. Data, reported as arbitrary units, are mean ± SEM of at least 3 distinct experiments.

P < .05 versus respective control

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