Quantitative RT-PCR analysis for the expression of TGF-β and of osteocalcin in the bone marrow from Gata1low mice harboring the mutation in the C57BL/6, DBA/2, or CD1 backgrounds, as indicated
. | . | TGF-β . | . | Osteocalcin . | . | ||
|---|---|---|---|---|---|---|---|
| Strain . | Genotype . | ΔCt . | 2—ΔΔCt . | ΔCt . | 2—ΔΔCt . | ||
| C57BL/6 | |||||||
| F1 + F2 | Wild type | 5.5 ± 1.1 | 1.2 ± 0.9 | 1.4 ± 0.3 | 1.0 ± 0.2 | ||
| F1 + F2 | Gata1low/+ | 6.7 ± 0.9 | 0.5 ± 0.3 | — 2.1 ± 0.5* | 12.4 ± 4.5* | ||
| DBA/2 | |||||||
| F1 + F2 | Wild type | 5.3 ± 1.0 | 1.1 ± 0.6 | 1.0 ± 0.2 | 1.0 ± 0.1 | ||
| F2 | Gata1low/0 | 7.4 ± 0.6* | 0.3 ± 0.1* | — 3.3 ± 0.41* | 19.0 ± 4.4* | ||
| CD1 | |||||||
| F1 + F2 | Wild type | 4.4 ± 0.9 | 1.1 ± 0.5 | 1.1 ± 0.4 | 1.0 ± 0.3 | ||
| F2 | Gata1low/0 | 8.5 ± 2.2* | 0.1 ± 0.1* | — 2.0 ± 0.8* | 9.3 ± 5.1* | ||
. | . | TGF-β . | . | Osteocalcin . | . | ||
|---|---|---|---|---|---|---|---|
| Strain . | Genotype . | ΔCt . | 2—ΔΔCt . | ΔCt . | 2—ΔΔCt . | ||
| C57BL/6 | |||||||
| F1 + F2 | Wild type | 5.5 ± 1.1 | 1.2 ± 0.9 | 1.4 ± 0.3 | 1.0 ± 0.2 | ||
| F1 + F2 | Gata1low/+ | 6.7 ± 0.9 | 0.5 ± 0.3 | — 2.1 ± 0.5* | 12.4 ± 4.5* | ||
| DBA/2 | |||||||
| F1 + F2 | Wild type | 5.3 ± 1.0 | 1.1 ± 0.6 | 1.0 ± 0.2 | 1.0 ± 0.1 | ||
| F2 | Gata1low/0 | 7.4 ± 0.6* | 0.3 ± 0.1* | — 3.3 ± 0.41* | 19.0 ± 4.4* | ||
| CD1 | |||||||
| F1 + F2 | Wild type | 4.4 ± 0.9 | 1.1 ± 0.5 | 1.1 ± 0.4 | 1.0 ± 0.3 | ||
| F2 | Gata1low/0 | 8.5 ± 2.2* | 0.1 ± 0.1* | — 2.0 ± 0.8* | 9.3 ± 5.1* | ||
The values obtained in age-matched littermates are reported for comparison. Results represent the mean (±SD) of the values determined for 3 individual mice per experimental group. Each mouse was assayed in triplicate. 2—ΔΔCt values were calculated using the mean value of the corresponding control as calibrator.
Values statistically different (< .05-.01) from those observed in the corresponding wild-type littermates