P falciparum invasion of band 3 Neapolis red cells
. | Parasitemia (%) . | . | . | ||
|---|---|---|---|---|---|
. | Invasion, 24 h . | Maturation, 48 h . | Reinvasion, 72 h . | ||
| Control | 5.80 ± 0.71 (100) | 3.65 ± 1.63 (100) | 5.20 ± 1.27 (100) | ||
| Mother | 4.35 ± 1.48 (75) | 2.55 ± 1.34 (70) | 4.20 ± 0.42 (81) | ||
| Proband | 0.75 ± 0.07 (13) | 0.20 ± 0.00 (5.5) | 0.10 ± 0.00 (1.9) | ||
. | Parasitemia (%) . | . | . | ||
|---|---|---|---|---|---|
. | Invasion, 24 h . | Maturation, 48 h . | Reinvasion, 72 h . | ||
| Control | 5.80 ± 0.71 (100) | 3.65 ± 1.63 (100) | 5.20 ± 1.27 (100) | ||
| Mother | 4.35 ± 1.48 (75) | 2.55 ± 1.34 (70) | 4.20 ± 0.42 (81) | ||
| Proband | 0.75 ± 0.07 (13) | 0.20 ± 0.00 (5.5) | 0.10 ± 0.00 (1.9) | ||
Cultures were inoculated by the addition of purified schizont-stage parasitized normal red cells (purity more than 95%) to test red cells suspended in growth medium (hematocrit, 0.5%). Two strains of parasite (Palo alto and FCR3) were used in each case. After 24 hours (ring stage of first cycle), 48 hours (trophozoite stage of the first cycle), and 72 hours (ring stage of second cycle) of culture, slides were prepared and stained with Diff-Quik parasite stain, and stage-dependent parasitemia was assessed by microscopic inspection. Values are expressed as percent of parasitized red cells during the course of the experiment. In parentheses, growth is normalized assuming 100% growth for the control.