Table 1. Identification by mass... | American Society of Hematology

Table 1.

Identification by mass spectrometry of adaptors and structural proteins involved in cytoskeleton organization in NPM-ALK–positive cells

NCBI accession no.	Identified protein	No. of peptide matches	Sequence coverage, %	IP	MW, kDa	MALDI identification	MS/MS identification
NP_0055382	p130 Crk-associated substrate (p130Cas)	3	6	P-Tyr	93	–	+
AAH08633	Actin, beta	9	29	P-Tyr	41	+	+
AAC16672	Alpha-actinin-2-associated LIM protein	6	21	P-Tyr	34	+	–
AAA61279	Vimentin	15	32	ALK	53	+	+
P05217	Tubulin, beta	9	28	ALK	48	+	+
AAC51654	Myosin VI	8	8	P-Tyr	147	+	+
NP_005955	Myosin heavy chain, nonmuscle type B	7	5	ALK	227	+	+
NP_002464	Myosin, heavy polypeptide 9, nonmuscle	22	16	ALK	229	+	–
NP_006650	Tubulin, gamma complex-associated protein 2	2	2	ALK	103	–	+
NP_004915	Actinin alpha 4	2	7	ALK	104	–	+
NP_055140	Coronin 1C	2	4	ALK	53	–	+
NP_002009	Flightless 1 homolog	3	2	ALK	144	–	+
NP_056065	Coiled-coil protein BICD2	7	10	P-Tyr	93	–	+
NP_006089	RACK1	3	9	P-Tyr	35	–	+

NCBI accession no.	Identified protein	No. of peptide matches	Sequence coverage, %	IP	MW, kDa	MALDI identification	MS/MS identification
NP_0055382	p130 Crk-associated substrate (p130Cas)	3	6	P-Tyr	93	–	+
AAH08633	Actin, beta	9	29	P-Tyr	41	+	+
AAC16672	Alpha-actinin-2-associated LIM protein	6	21	P-Tyr	34	+	–
AAA61279	Vimentin	15	32	ALK	53	+	+
P05217	Tubulin, beta	9	28	ALK	48	+	+
AAC51654	Myosin VI	8	8	P-Tyr	147	+	+
NP_005955	Myosin heavy chain, nonmuscle type B	7	5	ALK	227	+	+
NP_002464	Myosin, heavy polypeptide 9, nonmuscle	22	16	ALK	229	+	–
NP_006650	Tubulin, gamma complex-associated protein 2	2	2	ALK	103	–	+
NP_004915	Actinin alpha 4	2	7	ALK	104	–	+
NP_055140	Coronin 1C	2	4	ALK	53	–	+
NP_002009	Flightless 1 homolog	3	2	ALK	144	–	+
NP_056065	Coiled-coil protein BICD2	7	10	P-Tyr	93	–	+
NP_006089	RACK1	3	9	P-Tyr	35	–	+

IP indicates immunoprecipitation; MW, molecular weight.