Mapping of LANA1 epitopes using immunoreactive peptides 5-73 of KSHV LANA1 against BM9 and BM10
Peptide . | Position . | Amino acid sequence . | BM9 . | BM10 . |
|---|---|---|---|---|
| P7 | 11-27 | GRSTGAPLTRGSCRKRN | + | + |
| P8 | 16-32 | APLTRGSCRKRNRSPER | + | + |
| P9 | 21-37 | GSCRKRNRSPERCDLGD | - | + |
| P19 | 71-87 | FTSGLPAFVSSPTLPVA | + | - |
| P43 | 191-207 | LAPSTLRSLRKRRLSS | + | + |
Peptide . | Position . | Amino acid sequence . | BM9 . | BM10 . |
|---|---|---|---|---|
| P7 | 11-27 | GRSTGAPLTRGSCRKRN | + | + |
| P8 | 16-32 | APLTRGSCRKRNRSPER | + | + |
| P9 | 21-37 | GSCRKRNRSPERCDLGD | - | + |
| P19 | 71-87 | FTSGLPAFVSSPTLPVA | + | - |
| P43 | 191-207 | LAPSTLRSLRKRRLSS | + | + |
Scanning peptide ELISA shows that peptides including LANA1 amino acid residues 11 to 32 (peptides P7-P8) and 11 to 37 (P7-P9) are bound by BM9 and BM10, respectively; BM9 also showed reactivity to residues 71-87 (P19); and both scFvs reacted to residues 191 to 207 (P43).