Impact of TGZ on TLR ligand-induced chemokine and cytokine secretion
. | No TLR ligand . | . | + TLR2L . | . | + TLR3L . | . | + TLR4L . | . | + TLR7L . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | DMSO . | TGZ . | DMSO . | TGZ . | DMSO . | TGZ . | DMSO . | TGZ . | DMSO . | TGZ . | |||||
| RANTES (CCL5; pg/mL)* | < 1.0 | < 1.0 | 1.7 | < 1.0 | > 20 000 | 1673 | 6160 | 526 | < 1.0 | < 1.0 | |||||
| MIP-1α (CCL3; pg/mL)* | < 1.0 | < 1.0 | < 1.0 | < 1.0 | 209.5 | 71.0 | 316.0 | 157 | < 1.0 | < 1.0 | |||||
| MCP-1 (CCL2; pg/mL)† | 52.5 | 174.6 | 97.5 | 453.9 | 729.9 | 1646.6 | 1004.9 | 1464.8 | 89.2 | 387.4 | |||||
| IL-12 (pg/mL)† | < 0.1 | < 0.1 | 23.3 | < 0.1 | > 1000 | 380.5 | > 1000 | 443.6 | 367.5 | < 0.1 | |||||
| IL-6 (pg/mL)† | 0 | 0 | 4.5 | 1.9 | 1113.5 | 1469.8 | 1744.7 | 1695.8 | 7.2 | 0 | |||||
| IL-10 (pg/mL)* | 2 | < 1 | 14 | < 1 | 26 | < 1 | 224 | 5 | 5 | < 1 | |||||
. | No TLR ligand . | . | + TLR2L . | . | + TLR3L . | . | + TLR4L . | . | + TLR7L . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | DMSO . | TGZ . | DMSO . | TGZ . | DMSO . | TGZ . | DMSO . | TGZ . | DMSO . | TGZ . | |||||
| RANTES (CCL5; pg/mL)* | < 1.0 | < 1.0 | 1.7 | < 1.0 | > 20 000 | 1673 | 6160 | 526 | < 1.0 | < 1.0 | |||||
| MIP-1α (CCL3; pg/mL)* | < 1.0 | < 1.0 | < 1.0 | < 1.0 | 209.5 | 71.0 | 316.0 | 157 | < 1.0 | < 1.0 | |||||
| MCP-1 (CCL2; pg/mL)† | 52.5 | 174.6 | 97.5 | 453.9 | 729.9 | 1646.6 | 1004.9 | 1464.8 | 89.2 | 387.4 | |||||
| IL-12 (pg/mL)† | < 0.1 | < 0.1 | 23.3 | < 0.1 | > 1000 | 380.5 | > 1000 | 443.6 | 367.5 | < 0.1 | |||||
| IL-6 (pg/mL)† | 0 | 0 | 4.5 | 1.9 | 1113.5 | 1469.8 | 1744.7 | 1695.8 | 7.2 | 0 | |||||
| IL-10 (pg/mL)* | 2 | < 1 | 14 | < 1 | 26 | < 1 | 224 | 5 | 5 | < 1 | |||||
Peripheral blood adhering monocytes were incubated for 6 days in the presence of GM-CSF and IL-4 with or without 5 μM TGZ starting from the first day of culture. The cells were incubated with different TLR ligands as a maturation stimulus 24 hours before harvesting. Chemokine and cytokine secretion was determined in the supernatant using commercially available ELISAs.
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