Table 4.

Baseline (before transfusion) differences between patients with low and high ΔHb, as well as among RBC units used in their transfusion events

ΔHbpost-related variableLowHighP valueΔHbweek-related variableLowHighP value
A. Patients with TDT, baseline 
Complete blood count and biochemicals Complete blood count and biochemicals 
NEU, ×109/L 6.40 ± 3.38 3.97 ± 1.87 .033  Hb, g/dL 10.12 ± 0.84 9.19 ± 0.97 .007 
MON, 109/L 0.94 ± 0.57 0.54 ± 0.32 .024  HCT, % 31.07 ± 2.65 28.34 ± 2.98 .007 
NLR 3.00 ± 1.63 1.57 ± 0.61 .007  PT/INR 1.41 ± 0.58 1.16 ± 0.09 .036 
Free protein S (% of control) 49.1 ± 17.9 61.7 ± 12.5 .029  α2-Macroglobulin, mg/dL 161 ± 94 229 ± 77 .026 
VWF antigen (% of control) 102.9 ± 44.6 81.9 ± 22.3 .091  — — — — 
RBCs and EVs RBCs 
PS+ RBCs, % 1.26 ± 1.39 0.39 ± 0.33 .001  PS+ RBCs, % 1.17 ± 1.44 0.52 ± 0.50 .045 
EVs size, nm 202 ± 73 158 ± 42 .080  — — — — 
Plasma metabolites Plasma metabolites 
Adenosine 0.511 ± 1.241 0.325 ± 0.113 .041  5-Oxoproline 3.001 ± 0.606 3.365 ± 0.447 .045 
Glycocholic acid 2.594 ± 3.363 0.576 ± 0.480 .018  N-methyl-l-glutamic acid 0.100 ± 0.028 0.079 ± 0.028 .035 
L-methionine 0.096 ±0.080 0.174 ±0.106 .021  MiNP 0.014 ± 0.023 0.006 ± 0.012 .029 
B. RBC units 
Complete blood count Complete blood count 
PLT, 109/L 3.68 ±2.45 2.2 ± 1.7 .038  — — — — 
RBCs and EVs RBCs and EVs 
PS+RBC, % 0.25 ± 0.14 0.19 ± 0.16 .066  Oxidative hemolysis, mg/dL 14.9 ± 11.7 7.5 ± 5.3 .031 
TR-like PPA, RFU 40655 ± 10182 48410 ± 13056 .061 Osmotic hemolysis, 24 hours, MCF, NaCl% 0.506 ± 0.033 0.481 ± 0.034 .051 
Procoagulant phospholipid activity, % 13. 0 ± 7.0 7.3 ± 3.8 .032  CH-like PPA, m, RFU 31349 ± 15009 59887 ± 37743 .032 
Supernatant metabolites Supernatant metabolites 
Dimethyl-arginine 4.67 ± 1.10 4.29 ± 1.65 .079  L-Arginine 0.067± 0.108 0.117 ± 0.201 .065 
Dopamine 4.75 ± 2.10 3.81 ± 1.53 .084  L-Homocysteine 0.001 ± 0.003 0.004 ± 0.006 .045 
Norepinephrine 1.49 ± 0.62 1.19 ± 0.49 .022  Melatonin 1.41 ± 1.15 0.63 ± 0.83 .018 
Normetanephrine 31.82 ± 6.74 27.56 ± 7.77 .009  Thiamine monophosphate 0.084 ± 0.088 0.139 ± 0.087 .020 
Glutaryl-L-carnitine 4.46 ± 3.14 2.80 ± 2.59 .069  Phthalic acid 43.01 ± 28.92 29.58 ± 25.39 .017 
2-Amino-isobutyric acid 0.127 ± 0.042 0.102 ± 0.042 .038  MEHP 14.20 ± 8.76 10.13 ± 7.75 .020 
— — — —  MMP 0.290 ± 0.474 0.140 ± 0.248 .037 
— — — — d-Lactose 0.298 ± 0.122 0.259 ± 0.204 .037 
ΔHbpost-related variableLowHighP valueΔHbweek-related variableLowHighP value
A. Patients with TDT, baseline 
Complete blood count and biochemicals Complete blood count and biochemicals 
NEU, ×109/L 6.40 ± 3.38 3.97 ± 1.87 .033  Hb, g/dL 10.12 ± 0.84 9.19 ± 0.97 .007 
MON, 109/L 0.94 ± 0.57 0.54 ± 0.32 .024  HCT, % 31.07 ± 2.65 28.34 ± 2.98 .007 
NLR 3.00 ± 1.63 1.57 ± 0.61 .007  PT/INR 1.41 ± 0.58 1.16 ± 0.09 .036 
Free protein S (% of control) 49.1 ± 17.9 61.7 ± 12.5 .029  α2-Macroglobulin, mg/dL 161 ± 94 229 ± 77 .026 
VWF antigen (% of control) 102.9 ± 44.6 81.9 ± 22.3 .091  — — — — 
RBCs and EVs RBCs 
PS+ RBCs, % 1.26 ± 1.39 0.39 ± 0.33 .001  PS+ RBCs, % 1.17 ± 1.44 0.52 ± 0.50 .045 
EVs size, nm 202 ± 73 158 ± 42 .080  — — — — 
Plasma metabolites Plasma metabolites 
Adenosine 0.511 ± 1.241 0.325 ± 0.113 .041  5-Oxoproline 3.001 ± 0.606 3.365 ± 0.447 .045 
Glycocholic acid 2.594 ± 3.363 0.576 ± 0.480 .018  N-methyl-l-glutamic acid 0.100 ± 0.028 0.079 ± 0.028 .035 
L-methionine 0.096 ±0.080 0.174 ±0.106 .021  MiNP 0.014 ± 0.023 0.006 ± 0.012 .029 
B. RBC units 
Complete blood count Complete blood count 
PLT, 109/L 3.68 ±2.45 2.2 ± 1.7 .038  — — — — 
RBCs and EVs RBCs and EVs 
PS+RBC, % 0.25 ± 0.14 0.19 ± 0.16 .066  Oxidative hemolysis, mg/dL 14.9 ± 11.7 7.5 ± 5.3 .031 
TR-like PPA, RFU 40655 ± 10182 48410 ± 13056 .061 Osmotic hemolysis, 24 hours, MCF, NaCl% 0.506 ± 0.033 0.481 ± 0.034 .051 
Procoagulant phospholipid activity, % 13. 0 ± 7.0 7.3 ± 3.8 .032  CH-like PPA, m, RFU 31349 ± 15009 59887 ± 37743 .032 
Supernatant metabolites Supernatant metabolites 
Dimethyl-arginine 4.67 ± 1.10 4.29 ± 1.65 .079  L-Arginine 0.067± 0.108 0.117 ± 0.201 .065 
Dopamine 4.75 ± 2.10 3.81 ± 1.53 .084  L-Homocysteine 0.001 ± 0.003 0.004 ± 0.006 .045 
Norepinephrine 1.49 ± 0.62 1.19 ± 0.49 .022  Melatonin 1.41 ± 1.15 0.63 ± 0.83 .018 
Normetanephrine 31.82 ± 6.74 27.56 ± 7.77 .009  Thiamine monophosphate 0.084 ± 0.088 0.139 ± 0.087 .020 
Glutaryl-L-carnitine 4.46 ± 3.14 2.80 ± 2.59 .069  Phthalic acid 43.01 ± 28.92 29.58 ± 25.39 .017 
2-Amino-isobutyric acid 0.127 ± 0.042 0.102 ± 0.042 .038  MEHP 14.20 ± 8.76 10.13 ± 7.75 .020 
— — — —  MMP 0.290 ± 0.474 0.140 ± 0.248 .037 
— — — — d-Lactose 0.298 ± 0.122 0.259 ± 0.204 .037 

Independent t test or Mann-Whitney U (2 samples) test. Metabolites: normalized values.

Bold: higher values between high- and low-ΔHb groups.

CH, chymotrypsin; INR, international normalized ratio; m; membrane; MCF, mean corpuscular fragility; MiNP, monoisononyl phthalate; MON, monocyte; NEU, neutrophil; RFU, relative fluorescence units; TR, trypsin.

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