Table 2.

Apheresis parameters of published trials for SCD gene therapy

TrialPatients (reported)Premobilization transfusionApheresis cyclesApheresis starting material cell target (CD34+ cells per kilogram)Gene therapy product cell dose target (CD34+ cells per kilogram)Comments
Lovotibeglogene
autotemcel (bluebird)12  
15 60 days 8 patients, 1 cycle
6 patients, 2 cycles
1 patient, 3 cycles
1.7 apheresis/patient (2 d/cycle) 
15 × 106 3 × 106 Cell products shipped fresh 
Exagamglogene
autotemcel (Vertex)13  
44 8 weeks Median 2 per cycles
32% of patients ≥3 cycles of 2 days per cycle 
15 × 106 3.5 × 106 Cell products shipped fresh 
OTQ923 Novartis50  60 days Three patients with 3, 2, and 2 cycles, respectively, of 2-3 days per patient 15 × 106 2 × 106 Cryopreserved, 2/3 patients met cell dose target 
BCH-BB694 (BCH/NHLBI)14  12 weeks 1 cycle of up to 2 apheresis days 15 × 106 4 × 106 Cell products shipped fresh 
TrialPatients (reported)Premobilization transfusionApheresis cyclesApheresis starting material cell target (CD34+ cells per kilogram)Gene therapy product cell dose target (CD34+ cells per kilogram)Comments
Lovotibeglogene
autotemcel (bluebird)12  
15 60 days 8 patients, 1 cycle
6 patients, 2 cycles
1 patient, 3 cycles
1.7 apheresis/patient (2 d/cycle) 
15 × 106 3 × 106 Cell products shipped fresh 
Exagamglogene
autotemcel (Vertex)13  
44 8 weeks Median 2 per cycles
32% of patients ≥3 cycles of 2 days per cycle 
15 × 106 3.5 × 106 Cell products shipped fresh 
OTQ923 Novartis50  60 days Three patients with 3, 2, and 2 cycles, respectively, of 2-3 days per patient 15 × 106 2 × 106 Cryopreserved, 2/3 patients met cell dose target 
BCH-BB694 (BCH/NHLBI)14  12 weeks 1 cycle of up to 2 apheresis days 15 × 106 4 × 106 Cell products shipped fresh 

NHLBI, National Heart, Lung, and Blood Institute.

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