Practice variability among participating collection sites
| Survey queries and responses (N = 6) . | No. of responses (%) . |
|---|---|
| Patient age group | |
| Adults | 1 (16.7%) |
| Pediatrics | 1 (16.7%) |
| Adult and pediatrics | 4 (67%) |
| Pediatric patient age range | |
| 0-18 y | 2 (33.3%) |
| 0-21 y | 1 (16.7%) |
| 0-35 y | 1 (16.7%) |
| No response | 2 (33.3%) |
| Pre-collection RBC transfusion type | |
| Manual whole blood exchange | 1 (16.7%) |
| Simple RBC transfusion | 4 (66.7%) |
| Automated RCE | 6 (100%) |
| Pre-collection transfusion number of sessions | |
| Manual whole blood exchange | |
| 2 sessions | 1 (16.7%) |
| Simple RBC transfusion | |
| 0-1 sessions | 1 (16.7%) |
| 2-3 sessions | 1 (16.7%) |
| 3 sessions | 1 (16.7%) |
| 6 sessions | 1 (16.7%) |
| Automated RCE | |
| 1 sessions | 1 (16.7%) |
| 2 sessions | 1 (16.7%) |
| 2-3 sessions | 1 (16.7%) |
| 3 sessions | 2 (33.3%) |
| 3-5 sessions | 1 (16.7%) |
| Precollection RBC transfusion frequency | |
| 3 wk | 1 (16.7%) |
| 4 wk | 5 (83%) |
| Last precollection RBC transfusion prior to PBSC collection (d) | |
| 1 | 2 (33.3%) |
| 1-3 | 1 (16.7%) |
| 3 | 1 (16.7%) |
| 6 | 1 (16.7%) |
| 10 | 1 (16.7%) |
| Posttransfusion HbS percentage | |
| 5-15% | 1 (16.7%) |
| 15% | 2 (33.3%) |
| 22% | 1 (16.7%) |
| 30% | 2 (33.3%) |
| Apheresis collection instrument | |
| Spectra Optia | 6 (100%) |
| Fenwal Amicus | 0 |
| Spectra Optia protocol | |
| cMNC | 3 (50%) |
| MNC | 1 (16.7%) |
| No answer | 2 (33.3%) |
| Total blood volumes processed per procedure | |
| 4 total blood volumes | 3 (50%) |
| No answer | 3 (50%) |
| Plerixafor administration prior to collection (h) | |
| 2 h | 2 (33.3%) |
| 2-3 h | 1 (16.7%) |
| 4 h | 3 (50%) |
| Precollection peripheral blood CD34+ cell count on day 1 (CD34+ cells/μL) | |
| 0.2 | 1 (16.7%) |
| 25 | 1 (16.7%) |
| 30 | 2 (33.3%) |
| 35 | 1 (16.7%) |
| 41 | 1 (16.7%) |
| Anticoagulant: whole blood ratio | |
| 1:13 | 1 (16.7%) |
| 1:12 | 2 (33.3%) |
| 1:10 | 2 (33.3%) |
| No answer | 1 (16.7%) |
| Target color of the collect line | |
| Second lightest color | 1 (16.7%) |
| Second darkest color | 1 (16.7%) |
| Between the 2 darkest colors | 3 (50%) |
| No response | 1 (16.7%) |
| Collection efficiency (CE2 formula) | |
| 35 | 2 (33.3%) |
| 43 | 1 (16.7%) |
| 45 | 1 (16.7%) |
| No answer | 2 (33.3%) |
| Number of collection cycles | |
| 1 | 2 (33.3%) |
| 2 | 3 (50%) |
| No answer | 1 (16.7%) |
| CD34+ cells/kg yield per day | |
| 1 x 106 | 1 (16.7%) |
| 3 x 106 | 2 (33.3%) |
| 4 x 106 | 1 (16.7%) |
| 6.46 x 106 | 1 (16.7%) |
| No answer | 1 (16.7%) |
| Product TNC count | |
| 3 x 109 | 1 (16.7%) |
| 4 x 109 | 1 (16.7%) |
| No answer | 4 (66.7%) |
| CD34+ cell viability (%) | |
| 97% | 1 (16.7%) |
| 99% | 1 (16.7%) |
| 99-100% | 1 (16.7%) |
| 100% | 1 (16.7%) |
| No answer | 2 (50%) |
| Product hematocrit (%) | |
| 2% | 1 (16.7%) |
| 7% | 1 (16.7%) |
| 5-8% | 1 (16.7%) |
| 12% | 1 (16.7%) |
| No answer | 2 (33.3%) |
| TBV to process | |
| Fixed TBV | 2 (33.3%) |
| Fixed TBV or liters of whole blood | 1 (16.7%) |
| Fixed time | 2 (33.3%) |
| Prediction algorithm | 1 (16.7%) |
| Criteria to proceed with PBSC collection | |
| No | 4 (66.7%) |
| Yes | |
| Minimum peripheral blood CD34+ cell count | 1 (16.7%) |
| 10 | 1 (16.7%) |
| Collection parameter altered | |
| Collection preference | 4 (80%) |
| Increasing collection days | 1 (16.7%) |
| No answer | 1 (16.7%) |
| Survey queries and responses (N = 6) . | No. of responses (%) . |
|---|---|
| Patient age group | |
| Adults | 1 (16.7%) |
| Pediatrics | 1 (16.7%) |
| Adult and pediatrics | 4 (67%) |
| Pediatric patient age range | |
| 0-18 y | 2 (33.3%) |
| 0-21 y | 1 (16.7%) |
| 0-35 y | 1 (16.7%) |
| No response | 2 (33.3%) |
| Pre-collection RBC transfusion type | |
| Manual whole blood exchange | 1 (16.7%) |
| Simple RBC transfusion | 4 (66.7%) |
| Automated RCE | 6 (100%) |
| Pre-collection transfusion number of sessions | |
| Manual whole blood exchange | |
| 2 sessions | 1 (16.7%) |
| Simple RBC transfusion | |
| 0-1 sessions | 1 (16.7%) |
| 2-3 sessions | 1 (16.7%) |
| 3 sessions | 1 (16.7%) |
| 6 sessions | 1 (16.7%) |
| Automated RCE | |
| 1 sessions | 1 (16.7%) |
| 2 sessions | 1 (16.7%) |
| 2-3 sessions | 1 (16.7%) |
| 3 sessions | 2 (33.3%) |
| 3-5 sessions | 1 (16.7%) |
| Precollection RBC transfusion frequency | |
| 3 wk | 1 (16.7%) |
| 4 wk | 5 (83%) |
| Last precollection RBC transfusion prior to PBSC collection (d) | |
| 1 | 2 (33.3%) |
| 1-3 | 1 (16.7%) |
| 3 | 1 (16.7%) |
| 6 | 1 (16.7%) |
| 10 | 1 (16.7%) |
| Posttransfusion HbS percentage | |
| 5-15% | 1 (16.7%) |
| 15% | 2 (33.3%) |
| 22% | 1 (16.7%) |
| 30% | 2 (33.3%) |
| Apheresis collection instrument | |
| Spectra Optia | 6 (100%) |
| Fenwal Amicus | 0 |
| Spectra Optia protocol | |
| cMNC | 3 (50%) |
| MNC | 1 (16.7%) |
| No answer | 2 (33.3%) |
| Total blood volumes processed per procedure | |
| 4 total blood volumes | 3 (50%) |
| No answer | 3 (50%) |
| Plerixafor administration prior to collection (h) | |
| 2 h | 2 (33.3%) |
| 2-3 h | 1 (16.7%) |
| 4 h | 3 (50%) |
| Precollection peripheral blood CD34+ cell count on day 1 (CD34+ cells/μL) | |
| 0.2 | 1 (16.7%) |
| 25 | 1 (16.7%) |
| 30 | 2 (33.3%) |
| 35 | 1 (16.7%) |
| 41 | 1 (16.7%) |
| Anticoagulant: whole blood ratio | |
| 1:13 | 1 (16.7%) |
| 1:12 | 2 (33.3%) |
| 1:10 | 2 (33.3%) |
| No answer | 1 (16.7%) |
| Target color of the collect line | |
| Second lightest color | 1 (16.7%) |
| Second darkest color | 1 (16.7%) |
| Between the 2 darkest colors | 3 (50%) |
| No response | 1 (16.7%) |
| Collection efficiency (CE2 formula) | |
| 35 | 2 (33.3%) |
| 43 | 1 (16.7%) |
| 45 | 1 (16.7%) |
| No answer | 2 (33.3%) |
| Number of collection cycles | |
| 1 | 2 (33.3%) |
| 2 | 3 (50%) |
| No answer | 1 (16.7%) |
| CD34+ cells/kg yield per day | |
| 1 x 106 | 1 (16.7%) |
| 3 x 106 | 2 (33.3%) |
| 4 x 106 | 1 (16.7%) |
| 6.46 x 106 | 1 (16.7%) |
| No answer | 1 (16.7%) |
| Product TNC count | |
| 3 x 109 | 1 (16.7%) |
| 4 x 109 | 1 (16.7%) |
| No answer | 4 (66.7%) |
| CD34+ cell viability (%) | |
| 97% | 1 (16.7%) |
| 99% | 1 (16.7%) |
| 99-100% | 1 (16.7%) |
| 100% | 1 (16.7%) |
| No answer | 2 (50%) |
| Product hematocrit (%) | |
| 2% | 1 (16.7%) |
| 7% | 1 (16.7%) |
| 5-8% | 1 (16.7%) |
| 12% | 1 (16.7%) |
| No answer | 2 (33.3%) |
| TBV to process | |
| Fixed TBV | 2 (33.3%) |
| Fixed TBV or liters of whole blood | 1 (16.7%) |
| Fixed time | 2 (33.3%) |
| Prediction algorithm | 1 (16.7%) |
| Criteria to proceed with PBSC collection | |
| No | 4 (66.7%) |
| Yes | |
| Minimum peripheral blood CD34+ cell count | 1 (16.7%) |
| 10 | 1 (16.7%) |
| Collection parameter altered | |
| Collection preference | 4 (80%) |
| Increasing collection days | 1 (16.7%) |
| No answer | 1 (16.7%) |
cMNC, continuous mononuclear cell; MNC, mononuclear cell; SD, standard deviation; TBV, total blood volume; TNC, total nucleated cells.