Microcomputed tomography analysis of trabecular and cortical bone in 6-week-old control (Dmp1Cre) and Dmp1Cre.Socs3f/f mice treated with IgG or αLy6G for 2 weeks
| . | Dmp1Cre . | Dmp1Cre.Socs3f/f . | ANOVA result . | ||
|---|---|---|---|---|---|
| IgG . | αLy6G . | IgG . | αLy6G . | ||
| Sample number | 8 | 8 | 7 | 8 | |
| Trabecular bone volume (BV/TV) (%) | 3.76 ± 0.77 | 2.19 ± 0.40 | 11.01 ± 2.17∗∗∗ | 9.25 ± 0.74 | Genotype, <.0001; αLy6G, .1547; interaction, .9343 |
| Trabecular thickness (μm) | 60.80 ± 3.00 | 55.47 ± 1.91 | 73.81 ± 3.51∗∗∗ | 77.24 ± 1.77 | Genotype, <.0001; αLy6G, .7163; interaction, .1024 |
| Trabecular number (mm) | 0.61 ± 0.12 | 0.38 ± 0.06 | 1.45 ± 0.25∗∗∗ | 1.19 ± 0.08 | Genotype, <.0001; αLy6G, .0947; interaction, .9092 |
| Trabecular separation (μm) | 643.8 ± 46.8 | 720.9 ± 20.3† | 463.8 ± 39.4∗∗∗ | 536.8 ± 8.4† | Genotype, <.0001; αLy6G, .0270; interaction, .9503 |
| Metaphyseal cross-sectional area (mm2) | 2.28 ± 0.04 | 2.38 ± 0.04 | 2.16 ± 0.04∗ | 2.16 ± 0.03 | Genotype, .0002; αLy6G, .2071; interaction, .1782 |
| Metaphyseal cortical porosity (%) | 3.16 ± 0.71 | 1.86 ± 0.27 | 12.18 ± 0.94∗∗∗ | 12.95 ± 0.89 | Genotype, <.0001; αLy6G, .7304; interaction, .1776 |
| Low-density metaphyseal BV/TV (%) | 10.89 ± 0.31 | 10.22 ± 0.26 | 15.26 ± 0.55∗∗∗ | 15.20 ± 0.68 | Genotype, <.0001; αLy6G, .4566; interaction, .5298 |
| Mid-density metaphyseal BV/TV (%) | 13.83 ± 1.01 | 13.52 ± 1.23 | 17.97 ± 1.04∗∗∗ | 17.69 ± 0.94 | Genotype, .0005; αLy6G, .7805; interaction, .9906 |
| High-density metaphyseal BV/TV (%) | 10.01 ± 1.97 | 11.32 ± 2.32 | 13.93 ± 2.12 | 13.36 ± 2.13 | Genotype, .6657; αLy6G, .8646; interaction, .1783 |
| Diaphyseal cortical porosity (%) | 0.59 ± 0.17 | 0.26 ± 0.11 | 1.06 ± 0.39∗ | 1.18 ± 0.29 | Genotype, .0133; αLy6G, .6995; interaction, .3884 |
| Low-density diaphyseal BV/TV (%) | 7.74 ± 0.33 | 7.60 ± 0.38 | 8.36 ± 0.38 | 8.32 ± 0.37 | Genotype, .0754; αLy6G, .8020; interaction, .8899 |
| Mid-density diaphyseal BV/TV (%) | 10.02 ± 1.19 | 10.14 ± 1.19 | 11.20 ± 1.38 | 11.13 ± 1.33 | Genotype, .4007; αLy6G, .9839; interaction, .9421 |
| High-density diaphyseal BV/TV (%) | 26.67 ± 1.62 | 26.73 ± 2.21 | 30.33 ± 2.20 | 29.40 ± 2.59 | Genotype, .1584; αLy6G, .8419; interaction, .8216 |
| . | Dmp1Cre . | Dmp1Cre.Socs3f/f . | ANOVA result . | ||
|---|---|---|---|---|---|
| IgG . | αLy6G . | IgG . | αLy6G . | ||
| Sample number | 8 | 8 | 7 | 8 | |
| Trabecular bone volume (BV/TV) (%) | 3.76 ± 0.77 | 2.19 ± 0.40 | 11.01 ± 2.17∗∗∗ | 9.25 ± 0.74 | Genotype, <.0001; αLy6G, .1547; interaction, .9343 |
| Trabecular thickness (μm) | 60.80 ± 3.00 | 55.47 ± 1.91 | 73.81 ± 3.51∗∗∗ | 77.24 ± 1.77 | Genotype, <.0001; αLy6G, .7163; interaction, .1024 |
| Trabecular number (mm) | 0.61 ± 0.12 | 0.38 ± 0.06 | 1.45 ± 0.25∗∗∗ | 1.19 ± 0.08 | Genotype, <.0001; αLy6G, .0947; interaction, .9092 |
| Trabecular separation (μm) | 643.8 ± 46.8 | 720.9 ± 20.3† | 463.8 ± 39.4∗∗∗ | 536.8 ± 8.4† | Genotype, <.0001; αLy6G, .0270; interaction, .9503 |
| Metaphyseal cross-sectional area (mm2) | 2.28 ± 0.04 | 2.38 ± 0.04 | 2.16 ± 0.04∗ | 2.16 ± 0.03 | Genotype, .0002; αLy6G, .2071; interaction, .1782 |
| Metaphyseal cortical porosity (%) | 3.16 ± 0.71 | 1.86 ± 0.27 | 12.18 ± 0.94∗∗∗ | 12.95 ± 0.89 | Genotype, <.0001; αLy6G, .7304; interaction, .1776 |
| Low-density metaphyseal BV/TV (%) | 10.89 ± 0.31 | 10.22 ± 0.26 | 15.26 ± 0.55∗∗∗ | 15.20 ± 0.68 | Genotype, <.0001; αLy6G, .4566; interaction, .5298 |
| Mid-density metaphyseal BV/TV (%) | 13.83 ± 1.01 | 13.52 ± 1.23 | 17.97 ± 1.04∗∗∗ | 17.69 ± 0.94 | Genotype, .0005; αLy6G, .7805; interaction, .9906 |
| High-density metaphyseal BV/TV (%) | 10.01 ± 1.97 | 11.32 ± 2.32 | 13.93 ± 2.12 | 13.36 ± 2.13 | Genotype, .6657; αLy6G, .8646; interaction, .1783 |
| Diaphyseal cortical porosity (%) | 0.59 ± 0.17 | 0.26 ± 0.11 | 1.06 ± 0.39∗ | 1.18 ± 0.29 | Genotype, .0133; αLy6G, .6995; interaction, .3884 |
| Low-density diaphyseal BV/TV (%) | 7.74 ± 0.33 | 7.60 ± 0.38 | 8.36 ± 0.38 | 8.32 ± 0.37 | Genotype, .0754; αLy6G, .8020; interaction, .8899 |
| Mid-density diaphyseal BV/TV (%) | 10.02 ± 1.19 | 10.14 ± 1.19 | 11.20 ± 1.38 | 11.13 ± 1.33 | Genotype, .4007; αLy6G, .9839; interaction, .9421 |
| High-density diaphyseal BV/TV (%) | 26.67 ± 1.62 | 26.73 ± 2.21 | 30.33 ± 2.20 | 29.40 ± 2.59 | Genotype, .1584; αLy6G, .8419; interaction, .8216 |
Effect of genotype: ∗P < .05; ∗∗P < .01; ∗∗∗P < .001 vs Dmp1Cre IgG. Bold type indicates a significant P value.
BV/TV, bone volume/total volume.
†Effect of treatment: P value <.05 vs αLy6G of the same genotype, by 2-way ANOVA with Šidák multiple comparisons test.