Oligonucleotides used for mAb heavy chain Fc mutagenesis.
| Oligo ID . | Sequence (5’-3’) . | . | Reaction . | mAb variant . | Mutation . |
|---|---|---|---|---|---|
| BH110 | TCCTCAGCGTCGACCAAGGGCCCATCGGTCT | Forward | A | GASDALIE | — |
| BH113 | AGAGGAAGACGTCCGGTCCCGCCAGGAGTTCAGGTGCTGGG | Reverse | A | GASDALIE | G236A, S239D |
| BH112 | GAACTCCTGGCGGGACCGGACGTCTTCCTCTTCCCCCCAAA | Forward | B | GASDALIE | G236A, S239D |
| BH115 | TGGAGATGGTTTTCTCTTCGGGGAGTGGGAGGGCTTTGTTGGAGACC | Reverse | B | GASDALIE | I332E, A330L |
| BH114 | AAAGCCCTCCCACTCCCCGAAGAGAAAACCATCTCCAAAGCCAAAGG | Forward | C | GASDALIE | I332E, A330L |
| BH111 | CAGATCTGGATCCTCATCATTTACCCGGAGA | Reverse | C | GASDALIE | — |
| BH110 | TCCTCAGCGTCGACCAAGGGCCCATCGGTCT | Forward | A | GRLR | — |
| BH117 | GAGGAAGACTGACGGTCCCCTCAGGAGTTCAGGTGCTGGG | Reverse | A | GRLR | G236R |
| BH116 | GAACTCCTGAGGGGACCGTCAGTCTTCCTCTTCCCCCCAAA | Forward | B | GRLR | G236R |
| BH119 | CTCGATGGGGGCTGGGCGGGCTTTGTTGGAGACCTTGCACTTGTA | Reverse | B | GRLR | L328R |
| BH118 | AGGTCTCCAACAAAGCCCGCCCAGCCCCCATCGAGAAAACCATC | Forward | C | GRLR | L328R |
| BH111 | CAGATCTGGATCCTCATCATTTACCCGGAGA | Reverse | C | GRLR | — |
| Oligo ID . | Sequence (5’-3’) . | . | Reaction . | mAb variant . | Mutation . |
|---|---|---|---|---|---|
| BH110 | TCCTCAGCGTCGACCAAGGGCCCATCGGTCT | Forward | A | GASDALIE | — |
| BH113 | AGAGGAAGACGTCCGGTCCCGCCAGGAGTTCAGGTGCTGGG | Reverse | A | GASDALIE | G236A, S239D |
| BH112 | GAACTCCTGGCGGGACCGGACGTCTTCCTCTTCCCCCCAAA | Forward | B | GASDALIE | G236A, S239D |
| BH115 | TGGAGATGGTTTTCTCTTCGGGGAGTGGGAGGGCTTTGTTGGAGACC | Reverse | B | GASDALIE | I332E, A330L |
| BH114 | AAAGCCCTCCCACTCCCCGAAGAGAAAACCATCTCCAAAGCCAAAGG | Forward | C | GASDALIE | I332E, A330L |
| BH111 | CAGATCTGGATCCTCATCATTTACCCGGAGA | Reverse | C | GASDALIE | — |
| BH110 | TCCTCAGCGTCGACCAAGGGCCCATCGGTCT | Forward | A | GRLR | — |
| BH117 | GAGGAAGACTGACGGTCCCCTCAGGAGTTCAGGTGCTGGG | Reverse | A | GRLR | G236R |
| BH116 | GAACTCCTGAGGGGACCGTCAGTCTTCCTCTTCCCCCCAAA | Forward | B | GRLR | G236R |
| BH119 | CTCGATGGGGGCTGGGCGGGCTTTGTTGGAGACCTTGCACTTGTA | Reverse | B | GRLR | L328R |
| BH118 | AGGTCTCCAACAAAGCCCGCCCAGCCCCCATCGAGAAAACCATC | Forward | C | GRLR | L328R |
| BH111 | CAGATCTGGATCCTCATCATTTACCCGGAGA | Reverse | C | GRLR | — |