The effect of the G haplotype on thrombin generation was determined after initiation at 1 pM TF in NIHR BioResource participants
| CAT parameter . | Variable . | Median (IQR) . | Wilcoxon rank sum P value . | |
|---|---|---|---|---|
| F5-G/G (n = 7) . | F5-ref (n = 13) . | |||
| LT (min) | No Ab (t−) | 12.9 (10.2-20.2) | 9.25 (8.62-11.0) | .030∗ |
| Anti-TFPI (t+) | 3.36 (3.29-4.11) | 3.33 (3.13-3.70) | .781 (N.S.) | |
| Ratio (t−/t+) | 3.86 (3.29-5.52) | 2.80 (2.39-3.25) | .0085∗∗ | |
| Peak (nM thrombin) | No Ab | 31.4 (19.9-42.7) | 30.6 (20.3-49.7) | 1.00 (N.S.) |
| Anti-TFPI | 248 (234-282) | 239 (200-269) | .351 (N.S.) | |
| Ratio | 0.130 (0.0744-0.15) | 0.133 (0.0854-0.177) | .877 (N.S.) | |
| ttPeak (min) | No Ab | 17.6 (15.3-25.4) | 15.8 (14.0-16.2) | .115 (N.S.) |
| Anti-TFPI | 6.14 (5.78-7.15) | 6.03 (5.76-6.14) | .485 (N.S.) | |
| Ratio | 2.99 (2.86-3.74) | 2.57 (2.39-2.85) | .030∗ | |
| ETP (nM thrombin × min) | No Ab | 367 (256-508) | 410 (312-595) | .643 (N.S.) |
| Anti-TFPI | 1230 (1120-1350) | 1120 (930-1180) | .097 (N.S.) | |
| Ratio | 0.314 (0.195-0.441) | 0.388 (0.306-0.526) | .311 (N.S.) | |
| CAT parameter . | Variable . | Median (IQR) . | Wilcoxon rank sum P value . | |
|---|---|---|---|---|
| F5-G/G (n = 7) . | F5-ref (n = 13) . | |||
| LT (min) | No Ab (t−) | 12.9 (10.2-20.2) | 9.25 (8.62-11.0) | .030∗ |
| Anti-TFPI (t+) | 3.36 (3.29-4.11) | 3.33 (3.13-3.70) | .781 (N.S.) | |
| Ratio (t−/t+) | 3.86 (3.29-5.52) | 2.80 (2.39-3.25) | .0085∗∗ | |
| Peak (nM thrombin) | No Ab | 31.4 (19.9-42.7) | 30.6 (20.3-49.7) | 1.00 (N.S.) |
| Anti-TFPI | 248 (234-282) | 239 (200-269) | .351 (N.S.) | |
| Ratio | 0.130 (0.0744-0.15) | 0.133 (0.0854-0.177) | .877 (N.S.) | |
| ttPeak (min) | No Ab | 17.6 (15.3-25.4) | 15.8 (14.0-16.2) | .115 (N.S.) |
| Anti-TFPI | 6.14 (5.78-7.15) | 6.03 (5.76-6.14) | .485 (N.S.) | |
| Ratio | 2.99 (2.86-3.74) | 2.57 (2.39-2.85) | .030∗ | |
| ETP (nM thrombin × min) | No Ab | 367 (256-508) | 410 (312-595) | .643 (N.S.) |
| Anti-TFPI | 1230 (1120-1350) | 1120 (930-1180) | .097 (N.S.) | |
| Ratio | 0.314 (0.195-0.441) | 0.388 (0.306-0.526) | .311 (N.S.) | |
Thrombin generation was assessed by CAT after initiation by 1 pM TF in citrated plasma, collected in CTI, in the presence and absence of inhibitory anti-TFPI Abs. The LT, ttPeak, peak, and ETP of thrombin generation were calculated. The mean of 3 technical replicates of each parameter was calculated for each participant and then group medians were determined, which are represented in the table. The ratio for each parameter corresponds to the value determined by dividing the measurement in the absence of anti-TFPI Abs by that made in its presence. The values in parentheses next to the median values represent the lower and upper limits of the IQR. N.B. ∗P < .05; ∗∗P < .01.
CTI, corn trypsin inhibitor; ETP, endogenous thrombin potential; N.S., not significant; ttPeak, time to peak.