Comparison of the analytical sensitivity of in vitro assays on reference brain homogenates from BSE-infected sheep and a patient with vCJD
| Assay . | Seed . | Dilution of prion-infected brain homogenate . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 10−1 . | 10−2 . | 10−3 . | 10−4 . | 10−5 . | 10−6 . | 10−7 . | 10−8 . | 10−9 . | 10−10 . | 10−11 . | ||
| RT-QuIC∗ | vCJD | ND | ND | ND | 1 | 0.7 | 0.1 | 0 | 0 | 0 | 0.1 | ND |
| ShBSE | ND | ND | ND | 1 | 1 | 1 | 0.7 | 0.1 | 0 | 0 | ND | |
| mb-PMCA† | vCJD | ND | ND | ND | 1 | 1 | 1 | 1 | 0.3 | 0.1 | 0 | ND |
| ShBSE | ND | ND | ND | ND | 1 | 1 | 1 | 1 | 0.8 | 0.2 | 0 | |
| capture-PMCA‡ | vCJD | ND | ND | ND | ND | ND | 1 | 1 | 1 | 1 | 0.2 | 0.2 |
| ShBSE | ND | ND | ND | ND | 1 | 1 | 1 | 1 | 1 | 0.3 | ND | |
| Assay . | Seed . | Dilution of prion-infected brain homogenate . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 10−1 . | 10−2 . | 10−3 . | 10−4 . | 10−5 . | 10−6 . | 10−7 . | 10−8 . | 10−9 . | 10−10 . | 10−11 . | ||
| RT-QuIC∗ | vCJD | ND | ND | ND | 1 | 0.7 | 0.1 | 0 | 0 | 0 | 0.1 | ND |
| ShBSE | ND | ND | ND | 1 | 1 | 1 | 0.7 | 0.1 | 0 | 0 | ND | |
| mb-PMCA† | vCJD | ND | ND | ND | 1 | 1 | 1 | 1 | 0.3 | 0.1 | 0 | ND |
| ShBSE | ND | ND | ND | ND | 1 | 1 | 1 | 1 | 0.8 | 0.2 | 0 | |
| capture-PMCA‡ | vCJD | ND | ND | ND | ND | ND | 1 | 1 | 1 | 1 | 0.2 | 0.2 |
| ShBSE | ND | ND | ND | ND | 1 | 1 | 1 | 1 | 1 | 0.3 | ND | |
Data for RT-QuIC and mb-PMCA were published previously.
ND, not determined; ShBSE, sheep BSE; vCJD, variant Creutzfeldt-Jakob disease.
RT-QuIC data represent the mean proportion of positive replicates from 3 independent experiments.
mb-PMCA data represent the mean proportion of positive replicates from 2 or 3 independent experiments.
capture-PMCA data represent the mean proportion of positive replicates from 6 or 8 independent experiments for vCJD and ShBSE seeds spiked in human plasma or sheep buffy coat, respectively.