Mass spectrometry assays for measuring peripheral blood MRD
| Assay . | Type . | Platform . | Sensitivity . | Comments . |
|---|---|---|---|---|
| MASS-FIX (Mayo Clinic) | Intact light chain | MALDI-TOF | 0.01 g/dL | Commercially available through Mayo Clinic reference laboratory; has replaced SPEP/IFE in their workflow3; evaluated in STAMINA4 |
| EXENT (Thermo Fisher), previously known as QIP-MS) | Intact light chain | MALDI-TOF | 0.0015 g/dL | Commercially available in Europe; evaluated in, for example, GEM2012MENOS655 and GMMG-MM56 |
| M-Insight (Sebia) | Clonotypic peptide | LC-MS | 2 × 10−5 to 5 × 10−4 g/dL | Commercially available in the United States; evaluated in IFM20097,8 |
| EasyM (Rapid Novor) | Clonotypic peptide | LC-MS | 3 × 10−6 to 6 × 10−4 g/dL | Commercially available in the United States; evaluated in MCRN-0019 |
| Assay . | Type . | Platform . | Sensitivity . | Comments . |
|---|---|---|---|---|
| MASS-FIX (Mayo Clinic) | Intact light chain | MALDI-TOF | 0.01 g/dL | Commercially available through Mayo Clinic reference laboratory; has replaced SPEP/IFE in their workflow3; evaluated in STAMINA4 |
| EXENT (Thermo Fisher), previously known as QIP-MS) | Intact light chain | MALDI-TOF | 0.0015 g/dL | Commercially available in Europe; evaluated in, for example, GEM2012MENOS655 and GMMG-MM56 |
| M-Insight (Sebia) | Clonotypic peptide | LC-MS | 2 × 10−5 to 5 × 10−4 g/dL | Commercially available in the United States; evaluated in IFM20097,8 |
| EasyM (Rapid Novor) | Clonotypic peptide | LC-MS | 3 × 10−6 to 6 × 10−4 g/dL | Commercially available in the United States; evaluated in MCRN-0019 |
LC-MS, liquid chromatography mass spectrometry; MALDI-TOF, matrix-assisted laser desorption ionization–time of flight; QIP-MS, quantitative immunoprecipitation mass spectroscopy; SPEP/FIX, serum protein electrophoresis/serum immunofixation.