Expression of α-globin and β-globin in mouse and human blood vessels
| Blood vessel . | Detection approach . | α-globin . | β-globin . | Reference . |
|---|---|---|---|---|
| Mouse TD arteries | TEM of artery with immunostaining | Detected | Not assessed | 19 |
| Immunofluorescence analysis of sectioned artery | Detected | Not assessed in reference 1,2; not detected in reference 3 | 19,21,29 | |
| Immunofluorescence analysis of isolated ECs | Detected | Not detected | 19 | |
| Immunoblot of homogenized artery | Detected | Detected in reference 1; not in reference 2 | 19,21 | |
| RT-PCR analysis of ECs cocultured with VSMCs to recapitulate MEJs. | Detected | Not assessed | 19 | |
| RT-PCR analysis of whole artery | Detected | Not detected | 21 | |
| Mouse carotid arteries | Immunofluorescence analysis of sectioned artery | Detected | Not assessed | 19 |
| TEM of artery with immunostaining | Detected | Not detected | 19 | |
| RT-PCR analysis of whole artery | Detected | Not assessed | 19 | |
| Mouse aorta | Immunofluorescence analysis of sectioned artery | Not detected | Not detected | 29 |
| Human coronary artery | Immunoblot of ECs expanded in culture | Detected | Not detected | 19 |
| Immunoblot of ECs cocultured with VSMCs to recapitulate MEJs | Detected | Not detected | 19 | |
| RT-PCR and immunoblot of ECs overexpressing KLF2/KLF4 | Detected | Not detected (data now shown) | 30 | |
| Human mesenteric arterioles | IHC of artery section | Detected | Not detected | 21 |
| Human skeletal muscle arterioles | Immunofluorescence analysis of artery section | Detected | Not assessed | 19 |
| Human TD arteries | IHC of artery section | Detected | Detected | 19 |
| Human aorta | Immunoblot of homogenized artery | Not detected | Not detected | 31 |
| Human adipose tissue arteriole | Immunoblot of homogenized artery | Detected | Not detected | 31 |
| IHC of artery section | Detected | Not detected | 21,29 | |
| Human pulmonary artery | Immunoblot of homogenized artery | Detected | Detected | 32 |
| Blood vessel . | Detection approach . | α-globin . | β-globin . | Reference . |
|---|---|---|---|---|
| Mouse TD arteries | TEM of artery with immunostaining | Detected | Not assessed | 19 |
| Immunofluorescence analysis of sectioned artery | Detected | Not assessed in reference 1,2; not detected in reference 3 | 19,21,29 | |
| Immunofluorescence analysis of isolated ECs | Detected | Not detected | 19 | |
| Immunoblot of homogenized artery | Detected | Detected in reference 1; not in reference 2 | 19,21 | |
| RT-PCR analysis of ECs cocultured with VSMCs to recapitulate MEJs. | Detected | Not assessed | 19 | |
| RT-PCR analysis of whole artery | Detected | Not detected | 21 | |
| Mouse carotid arteries | Immunofluorescence analysis of sectioned artery | Detected | Not assessed | 19 |
| TEM of artery with immunostaining | Detected | Not detected | 19 | |
| RT-PCR analysis of whole artery | Detected | Not assessed | 19 | |
| Mouse aorta | Immunofluorescence analysis of sectioned artery | Not detected | Not detected | 29 |
| Human coronary artery | Immunoblot of ECs expanded in culture | Detected | Not detected | 19 |
| Immunoblot of ECs cocultured with VSMCs to recapitulate MEJs | Detected | Not detected | 19 | |
| RT-PCR and immunoblot of ECs overexpressing KLF2/KLF4 | Detected | Not detected (data now shown) | 30 | |
| Human mesenteric arterioles | IHC of artery section | Detected | Not detected | 21 |
| Human skeletal muscle arterioles | Immunofluorescence analysis of artery section | Detected | Not assessed | 19 |
| Human TD arteries | IHC of artery section | Detected | Detected | 19 |
| Human aorta | Immunoblot of homogenized artery | Not detected | Not detected | 31 |
| Human adipose tissue arteriole | Immunoblot of homogenized artery | Detected | Not detected | 31 |
| IHC of artery section | Detected | Not detected | 21,29 | |
| Human pulmonary artery | Immunoblot of homogenized artery | Detected | Detected | 32 |
IHC, immunohistochemistry; TD, thoracodorsal; TEM, transmission electron microscopy; RT-PCR, reverse transcription polymerase chain reaction.